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Production of interleukin (IL)-10 and IL-12 by murine colonic dendritic cells in response to microbial stimuli

Rigby, R J and Knight, S C and Kamm, M A and Stagg, A J (2005) Production of interleukin (IL)-10 and IL-12 by murine colonic dendritic cells in response to microbial stimuli. Clinical and Experimental Immunology, 139 (2). pp. 245-256. ISSN 0009-9104

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Abstract

Intestinal dendritic cells (DC) are likely to regulate immunity to gut microflora, but little is known about their responses to bacterial antigens. Therefore, DC from normal murine colon were characterized and their cytokine responses to components of Gram-negative and/or Gram-positive bacteria assessed. Cells were obtained by digestion of colonic tissue and contained DC that were identified by flow cytometry as CD11c(+) major histocompatibility complex (MHC) class II+ cells. Purified DC were obtained by immunomagnetic separation plus cell sorting. DC had the morphology of immature myeloid cells, were endocytically active, expressed low levels of co-stimulatory molecules and stimulated a weak allogeneic mixed leucocyte reaction. Analysis of flow cytometry data by a sensitive subtraction method allowed measurement of production of interleukin (IL)-12 and IL-10 by small numbers of gut DC by intracellular staining. Fewer than 5% of unstimulated DC produced either IL-10 or IL-12. IL-10 production was significantly up-regulated following stimulation with Bifidobacteria longum, but not after exposure to lipopolysaccharide (LPS) or Streptococcus faecium. In contrast, colonic DC produced IL-12 in response to both LPS and B.longum. Thus, colonic DC can produce both IL-12 and IL-10 following bacterial stimulation. Cell wall components from different bacteria stimulate distinct responses and may direct immune responses differentially in the gut.

Item Type: Article
Journal or Publication Title: Clinical and Experimental Immunology
Uncontrolled Keywords: cytokines ; dendritic cells ; mucosa ; rodent ; LIGAND-TREATED MICE ; T-CELLS ; IN-VIVO ; PEYERS PATCH ; COMMENSAL BACTERIA ; LAMINA PROPRIA ; ANTIGEN ; EXPRESSION ; INDUCTION ; IMMUNITY
Subjects:
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 49916
Deposited By: ep_importer_pure
Deposited On: 20 Sep 2011 16:18
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 19:33
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/49916

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