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Expansion of intestinal stem cells associated with long-term adaptation following ileocecal resection in mice

Dekaney, Christopher M. and Fong, Jerry J. and Rigby, Rachael J. and Lund, P. Kay and Henning, Susan J. and Helmrath, Michael A. (2007) Expansion of intestinal stem cells associated with long-term adaptation following ileocecal resection in mice. American Journal of Physiology-Gastrointestinal and Liver Physiology, 293 (5). G1013-G1022. ISSN 0193-1857

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Abstract

Sustained increases in mucosal surface area occur in remaining bowel following massive intestinal loss. The mechanisms responsible for expanding and perpetuating this response are not presently understood. We hypothesized that an increase in the number of intestinal stem cells ( ISC) occurs following intestinal resection and is an important component of the adaptive response in mice. This was assessed in the jejunum of mice 2 - 3 days, 4 - 5 days, 6 - 7 days, 2 wk, 6 wk, and 16 wk following ileocecal resection ( ICR) or sham operation. Changes in ISC following ICR compared with sham resulted in increased crypt fission and were assayed by 1) putative ISC population ( SP) by flow cytometry, 2) Musashi- 1 immunohistochemistry, and 3) bromodeoxyuridine ( BrdU) label retention. Observed early increases in crypt depth and villus height were not sustained 16 wk following operation. In contrast, long- term increases in intestinal caliber and overall number of crypts per circumference appear to account for the enhanced mucosal surface area following ICR. Flow cytometry demonstrated that significant increases in SP cells occur within 2 - 3 days following resection. By 7 days, ICR resulted in marked increases in crypt fission and Musashi- 1 immunohistochemistry staining. Separate label- retention studies confirmed a 20- fold increase in BrdU incorporation 6 wk following ICR, confirming an overall increase in the number of ISC. These studies support that expansion of ISC occurs following ICR, leading to an overall increase number of crypts through a process of fission and intestinal dilation. Understanding the mechanism expanding ISCs may provide important insight into management of intestinal failure.

Item Type: Article
Journal or Publication Title: American Journal of Physiology-Gastrointestinal and Liver Physiology
Uncontrolled Keywords: intestinal stem cells ; intestinal resection ; crypt fission ; label retention ; SMALL-BOWEL RESECTION ; TRANSGENIC MICE ; BETA-CATENIN ; MOUSE ; EPITHELIUM ; RAT ; PROLIFERATION ; HYPERPLASIA ; GROWTH ; CRYPT
Subjects:
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 49913
Deposited By: ep_importer_pure
Deposited On: 20 Sep 2011 16:14
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 19:33
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/49913

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