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Characterisation of oligosaccharides from the chondroitin/dermatan sulphates:1H and 13C NMR studies of oligosaccharides generated by nitrous acid depolymerisation

Lauder, Robert M. and Huckerby, Thomas and Nieduszynski, Ian and Sadler, Ian H. (2011) Characterisation of oligosaccharides from the chondroitin/dermatan sulphates:1H and 13C NMR studies of oligosaccharides generated by nitrous acid depolymerisation. Carbohydrate Research, 346 (14). pp. 2222-2227. ISSN 1873-426X

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Abstract

The polymers chondroitin sulphate and dermatan sulphate have been fragmented by an anhydrous hydrazine/nitrous acid procedure. The resulting disaccharides from the polymer repeat sequences were reduced with NaBH4 and purified by ion exchange chromatography. Whereas enzymatic depolymerisation leads to the loss of the distinction between glucuronic and iduronic acids of CS and DS in the resultant disaccharides, nitrous acid depolymerisation retains these structures. Complete 1H and 13C NMR data have been derived for the major components which were shown to have the structures: GlcA(b1?3)- anTal6S-ol (I) and L-IdoA(a1?3)anTal4S-ol (II), where anTal-ol represents (2,5)anhydro-D-talitol and 6S/4S represent O-ester sulphate groups at C-6/C-4 sites.

Item Type: Article
Journal or Publication Title: Carbohydrate Research
Uncontrolled Keywords: Glycosaminoglycan ; Chondroitin sulphate/sulfate ; NMR spectroscopy
Subjects: R Medicine > R Medicine (General)
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 49555
Deposited By: ep_importer_pure
Deposited On: 31 Aug 2011 09:13
Refereed?: Yes
Published?: Published
Last Modified: 26 Mar 2013 16:02
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/49555

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