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Use of green fluorescent protein in visualisation of pneumococcal invasion of broncho-epithelial cells in vivo

Kadioglu, A and Sharpe, J A and Lazou, I and Svanborg, C and Ockleford, C and Mitchell, T J and Andrew, P W (2001) Use of green fluorescent protein in visualisation of pneumococcal invasion of broncho-epithelial cells in vivo. FEMS Microbiology Letters, 194 (1). pp. 105-10. ISSN 0378-1097

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Abstract

The pneumococcus is the principle cause of bacterial pneumonia and also a major cause of bacterial meningitis. The mechanisms and sites of pneumococcal adherence and invasion of the respiratory tract in vivo are not clear however. We have made pneumococci expressing green fluorescent protein (GFP) and used it to trace pneumococcal adherence and invasion in vivo. By using GFP pneumococci we have shown bacterial adherence and invasion of broncho-epithelial cells in vivo by 4 h post-infection, with increases in pneumococcal invasiveness by 24 h. Using confocal image analysis we have shown varying levels of pneumococcal penetration and internalisation into host cells, as well as translocation through epithelial layers. To our knowledge this is the first report of pneumococcal invasion and cellular translocation in vivo.

Item Type: Article
Journal or Publication Title: FEMS Microbiology Letters
Uncontrolled Keywords: Animals ; Bacterial Adhesion ; Blood ; Bronchi ; Epithelial Cells ; Female ; Green Fluorescent Proteins ; Luminescent Proteins ; Lung ; Mice ; Microscopy, Confocal ; Plasmids ; Pneumococcal Infections ; Streptococcus pneumoniae ; Transformation, Bacterial
Subjects: UNSPECIFIED
Departments: Faculty of Health and Medicine > Medicine
ID Code: 49324
Deposited By: ep_importer_pure
Deposited On: 24 Aug 2011 11:11
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 19:27
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/49324

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