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Propidium iodide as a nuclear marker in immunofluorescence. I. Use with tissue and cytoskeleton studies

OCKLEFORD, C D and HSI, B L and WAKELY, J and BADLEY, R A and WHYTE, A and FAULK, W P (1981) Propidium iodide as a nuclear marker in immunofluorescence. I. Use with tissue and cytoskeleton studies. Journal of Immunological Methods, 43 (3). pp. 261-267.

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Abstract

Some examples are given of immunofluorescence with tissue sections and microtubular cytoskeletons of cultured cells where the fluorescent dye propidium iodide (PI) has been used as marker of nuclei. The emission wave length of PI is longer than that of flourescein, making it possible to use several different and commonly available filter combinations. The use of nuclei as positional indicators is often a more suitable method than phase microscopy combined with immunofluorescence because of low background illumination against which morphology is viewed, circumventing the need for often expensive phase optics.

Item Type: Article
Journal or Publication Title: Journal of Immunological Methods
Subjects: UNSPECIFIED
Departments: Faculty of Health and Medicine > Medicine
ID Code: 49288
Deposited By: ep_importer_pure
Deposited On: 05 Aug 2011 10:25
Refereed?: Yes
Published?: Published
Last Modified: 09 Apr 2014 22:39
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/49288

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