OCKLEFORD, C D and HSI, B L and WAKELY, J and BADLEY, R A and WHYTE, A and FAULK, W P (1981) Propidium iodide as a nuclear marker in immunofluorescence. I. Use with tissue and cytoskeleton studies. Journal of Immunological Methods, 43 (3). pp. 261-267.Full text not available from this repository.
Some examples are given of immunofluorescence with tissue sections and microtubular cytoskeletons of cultured cells where the fluorescent dye propidium iodide (PI) has been used as marker of nuclei. The emission wave length of PI is longer than that of flourescein, making it possible to use several different and commonly available filter combinations. The use of nuclei as positional indicators is often a more suitable method than phase microscopy combined with immunofluorescence because of low background illumination against which morphology is viewed, circumventing the need for often expensive phase optics.
|Journal or Publication Title:||Journal of Immunological Methods|
|Departments:||Faculty of Health and Medicine > Medicine|
|Deposited On:||05 Aug 2011 10:25|
|Last Modified:||26 Jul 2012 19:26|
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