BRIGHT, N A and OCKLEFORD, C D (1995) ULTRASTRUCTURAL DISTRIBUTION OF ENDOGENOUS IMMUNOGLOBULIN-G IN HUMAN TERM AMNIOCHORION. Cell and Tissue Research, 281 (2). pp. 367-374. ISSN 0302-766XFull text not available from this repository.
Maternal immunoglobulin-G (IgG) is known to be transported across the placental syncytiotrophoblast during the period when the human fetus is incapable of manufacturing these defensive molecules. In this study we investigated the possible role of the amniochorion, that surrounds the amniotic cavity in which the fetus lies, in the transfer of immunoglobulin. Endogenous IgG was localised in the amniochorion by confocal immunofluorescence microscopy and by ultrastructural labelling of ultrathin frozen tissue sections using the protein A-gold technique. Immunoreactivity was identified in the extracellular matrix tissues and necrotic amniotic epithelial cells. Healthy amniotic epithelial cells and cytotrophoblast cells of the chorion laeve were devoid of endogenous IgG. These results suggest a possible nonspecific paracellular transport pathway between cytotrophoblast cells, which may conceivably contribute to the acquisition of passive immunity by the fetus, and offer a rational explanation for the presence of small quantities of maternal IgG in the amniotic fluid.
|Journal or Publication Title:||Cell and Tissue Research|
|Uncontrolled Keywords:||PLACENTA ; AMNIOCHORION ; CYTOTROPHOBLAST CELLS ; IMMUNOGLOBULIN-G ; IMMUNOCYTOCHEMISTRY ; HUMAN ; ULTRATHIN FROZEN-SECTIONS ; PLACENTAL-TRANSFER ; IGG SUBCLASSES|
|Departments:||Faculty of Health and Medicine > Medicine|
|Deposited On:||04 Aug 2011 13:44|
|Last Modified:||07 Jan 2015 16:49|
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