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Leishmania species:detection and identification by nested PCR assay from skin samples of rodent reservoirs

Akhavan, Amir Ahmad and Mirhendi, Hossein and Khamesipour, Ali and Alimohammadian, Mohammad Hossein and Rassi, Yavar and Bates, Paul and Kamhawi, Shaden and Valenzuela, Jesus G and Arandian, Mohammad Hossein and Abdoli, Hamid and Jalali-zand, Niloufar and Jafari, Reza and Shareghi, Niloufar and Ghanei, Maryam and Yaghoobi-Ershadi, Mohammad Reza (2010) Leishmania species:detection and identification by nested PCR assay from skin samples of rodent reservoirs. Experimental Parasitology, 126 (4). pp. 552-556.

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Abstract

Many rodent species act as reservoir hosts of zoonotic cutaneous leishmaniasis in endemic areas. In the present study a simple and reliable assay based on nested PCR was developed for the detection and identification of Leishmania parasites from rodent skin samples. We designed Leishmania-specific primers that successfully amplified ITS regions of Leishmania major, Leishmania gerbilli and Leishmania turanica using nested PCR. Out of 95 field collected Rhombomys opimus, 21 were positive by microscopic examination and 48 by nested PCR. The percentage of gerbils infected with L. major, L. gerbilli and L. turanica was 3.2%, 1.1% and 27.4%, respectively. In 15.8% of the rodents, we found mixed natural infections by L. major and L. turanica, 1.1% by L. major and L. gerbilli, and 2.1% by the three species. We concluded that this method is simple and reliable for detecting and identifying Leishmania species circulating in rodent populations.

Item Type: Article
Journal or Publication Title: Experimental Parasitology
Uncontrolled Keywords: Animals ; DNA Primers ; DNA, Protozoan ; Disease Reservoirs ; Electrophoresis, Agar Gel ; Gerbillinae ; Iran ; Leishmania ; Leishmaniasis, Cutaneous ; Polymerase Chain Reaction ; Rodent Diseases ; Skin
Subjects: Q Science > QR Microbiology > QR355 Virology
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 49221
Deposited By: ep_importer_pure
Deposited On: 27 Jul 2011 14:54
Refereed?: Yes
Published?: Published
Last Modified: 06 Aug 2012 08:56
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/49221

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