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SYNCHROTRON X-RAY-DIFFRACTION STUDIES OF THE CORNEA, WITH IMPLICATIONS FOR STROMAL HYDRATION

MEEK, K M and FULLWOOD, N J and COOKE, P H and ELLIOTT, G F and MAURICE, D M and QUANTOCK, A J and WALL, R S and WORTHINGTON, C R (1991) SYNCHROTRON X-RAY-DIFFRACTION STUDIES OF THE CORNEA, WITH IMPLICATIONS FOR STROMAL HYDRATION. Biophysical Journal, 60 (2). pp. 467-474. ISSN 0006-3495

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Abstract

The intermolecular and interfibrillar spacings of collagen in bovine corneal stroma have been measured as a function of tissue hydration. Data were recorded from low- and high-angle x-ray diffraction patterns obtained using a high intensity synchrotron source. The most frequently occurring interfibrillar spacing varied from 34 nm in dry corneas to 76 nm at H = 5 (the hydration, H, is defined as the ratio of the weight of water to the dry weight). The most frequently occurring intermolecular Bragg spacing increased from 1.15 nm (dry) to approximately 1.60 nm at normal hydration (H almost-equal-to 3.2) and continued to increase only slowly above normal hydration. Most of the increase in the intermolecular spacing occurred between H = 0 and H = 1. Over this hydration range the interfibrillar and intermolecular spacings moved in tandem, which suggests that the initial water goes equally within and between the fibrils. Above H = 1 water goes preferentially between the fibrils. The results suggest that, even at normal hydration, water does not fill the interfibrillar space uniformly, and a proportion is located in another space or compartment. In dried-then-rehydrated corneas, a larger proportion ot the water goes into this other compartment. In both cases, it is possible to postulate a second set or population of fibrils that are more widely and irregularly separated and therefore do not contribute significantly to the diffraction pattern.

Item Type: Article
Journal or Publication Title: Biophysical Journal
Uncontrolled Keywords: COLLAGEN FIBRILS ; ELECTRON-MICROSCOPY
Subjects:
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 49176
Deposited By: ep_importer_pure
Deposited On: 22 Aug 2011 16:53
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 19:25
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/49176

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