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Keratan sulphate in the trabecular meshwork and cornea

Davis, Y and Fullwood, N J and Marcyniuk, B and Bonshek, R and Tullo, A and Nieduszynski, I A (1997) Keratan sulphate in the trabecular meshwork and cornea. Current Eye Research, 16 (7). pp. 677-686.

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Abstract

Purpose. A study was made of the distribution of keratan sulphate in the human anterior chamber. Methods. The monoclonal antibody, 5-D-4, was used in immuno-electron microscopy to visualise keratan sulphate distribution in the anterior chamber of 16 normal eyes, 7 Fuchs' dystrophy corneas, and a macular dystrophy cornea. Results. Keratan sulphate was detected in normal human aqueous humour and also on the surface of trabecular cells in the uveal meshwork. Normal corneal stroma showed an increase in keratan sulphate labelling from anterior to posterior, with marked labelling in the posterior region of Descemet's membrane. The apical surface of the corneal endothelium labelled positively, but showed considerable variation in the level of labelling from cell to cell. The macular dystrophy cornea had the classic histopathological features of a type I case, including a highly abnormal Descemet's membrane. No keratan sulphate was detected in the macular dystrophy patient's corneal stroma or serum. The Fuchs' endothelial dystrophy corneas showed a normal distribution of keratan sulphate labelling in the stroma. The Fuchs' endothelial cells labelled for keratan sulphate but were highly abnormal in appearance, often exhibiting long filopodia and appearing to be actively migrating. Conclusions. This work has shown that keratan sulphate has a much wider distribution than was previously believed. The detection of keratan sulphate on the trabecular and endothelial cell surfaces also suggests a possible role for this molecule in cell adhesion and/or migration.

Item Type: Article
Journal or Publication Title: Current Eye Research
Uncontrolled Keywords: cornea ; glycosaminoglycan ; electron microscopy ; keratan sulphate ; trabecular meshwork ; human ; SULFATE PROTEOGLYCAN ; DYSTROPHY ; COLLAGEN ; GLYCOSAMINOGLYCANS ; OLIGOSACCHARIDES ; TISSUE
Subjects: UNSPECIFIED
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 49170
Deposited By: ep_importer_pure
Deposited On: 22 Aug 2011 16:34
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 19:25
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/49170

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