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Human corneal keratan sulfates

Tai, G H and Nieduszynski, I A and Fullwood, N J and Huckerby, T N (1997) Human corneal keratan sulfates. Journal of Biological Chemistry, 272 (45). pp. 28227-28231.

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Abstract

The keratan sulfate-containing proteoglycans were isolated from fourteen pooled human corneas (thirteen from 61- to 86-year-olds, plus one from a 12-year-old). These proteoglycans were subjected to digestion with the enzyme keratanase II, and the released oligosaccharides, which included nonreducing termini and repeat region oligosaccharides but not linkage regions, were reduced with alkaline borohydride and identified on two separate ion-exchange columns. Both of the latter had been calibrated with samples, most of which had been derived from bovine corneal keratan sulfate (Tai, G.-H., Huckerby, T. N., and Nieduszynski, I. A. (1996) J. Biol, Chem. 271, 23535-23546) and all of which had been fully characterized by NMR spectroscopic analysis, The capping structures identified in human corneal keratan sulfates occurred in the relative proportions: NeuAc alpha(2-6)- >NeuAc alpha(2-3)- >GaINAc(S)beta(1-3)-. The other groups of capping structures which had been identified in bovine corneal keratan sulfate, i.e. NeuGc alpha(2-3)-, NeuGc alpha(2-6)-, GlcNAc(S)beta(1-3)- were absent, although the possibility of the presence of some Gal alpha(1-3)-structures could not be excluded. In addition, the human sample showed significantly higher levels of alpha(1-3) fucosylated repeat region structures than did the bovine sample, and it is not clear whether this reflects a species or age dependence as the bovine corneas were from young animals, whereas the human corneas were predominantly from an older group. The charge densities and keratan sulfate chain sizes of the human and bovine keratan sulfate-containing proteoglycans were seen to be similar.

Item Type: Article
Journal or Publication Title: Journal of Biological Chemistry
Uncontrolled Keywords: CORE PROTEIN ; II DIGESTION ; CUPROMERONIC BLUE ; LINKAGE-REGION ; OLIGOSACCHARIDES ; PROTEOGLYCAN ; CHAIN ; CHROMATOGRAPHY ; DISACCHARIDE
Subjects: UNSPECIFIED
Departments: Faculty of Science and Technology > Lancaster Environment Centre
Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 49169
Deposited By: ep_importer_pure
Deposited On: 22 Aug 2011 16:24
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 19:25
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/49169

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