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Imaging sclera with hard X-ray microscopy

Fullwood, Nigel J. and Martin, Frank and Bentley, Adam and Lee, Jin Pyung and Lee, Sang Joon (2011) Imaging sclera with hard X-ray microscopy. Micron, 42 (5). pp. 506-511. ISSN 0968-4328

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Abstract

In this study, the organization of collagen fibrils within the sclera of the eye was investigated using the 7 keV hard X-ray microscope of the Pohang light source and compared to images from electron and atomic force microscopy. From the captured X-ray images, individual collagen fibrils were observed clearly in a spatial resolution much better than 100 nm, both in longitudinal sections and in transverse sections. Some of the collagen fibrils showed evidence of axial periodicity. In some regions of the samples, we could see cross-bridge like structures between adjacent collagen fibrils. The X-ray microscope also allowed the observation of keratocytes and the lamella structure of the scleral stroma. The X-ray microscope has some unique advantages in the nano-scale imaging of bio-samples relative to other established imaging techniques. (C) 2011 Elsevier Ltd. All rights reserved.

Item Type: Article
Journal or Publication Title: Micron
Uncontrolled Keywords: X-ray microscopy ; Synchrotron ; Sclera ; Collagen ; Proteoglycans ; OBSERVING TRANSPARENT SPECIMENS ; PHASE-CONTRAST ; ELECTRON-MICROSCOPY ; CORNEAL STROMA ; RESOLUTION ; COLLAGENS ; AGGRECAN
Subjects: UNSPECIFIED
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 49094
Deposited By: ep_importer_pure
Deposited On: 21 Jul 2011 16:32
Refereed?: Yes
Published?: Published
Last Modified: 17 Sep 2013 08:26
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/49094

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