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The structure of the keratan sulphate chains attached to fibromodulin from human articular cartilage

Lauder, R M and Huckerby, T N and Nieduszynski, I A (1997) The structure of the keratan sulphate chains attached to fibromodulin from human articular cartilage. Glycoconjugate journal, 14 (5). pp. 651-660. ISSN 0282-0080

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Abstract

The small keratan sulphate proteoglycan, fibromodulin, has been isolated from pooled human articular cartilage. The main chain repeat region and the chain caps from the attached N-linked keratan sulphate chains have been fragmented by keratanase II digestion, and the oligosaccharides generated have been reduced and isolated. Their structures and abundance have been determined by high pH anion-exchange chromatography. These regions of the keratan sulphate from human articular cartilage fibromodulin have been found to have the following general structure: [GRAPHICS] Significantly, both alpha(2-6)- and alpha(2-3)-linked N-acetyl-neuraminic acid have been found in the capping oligosaccharides. Fucose, which is alpha(1-3)-linked as a branch to N-acetylglucosamine, has also been found along the length of the repeat region and in the capping region. The chains, which have been found to be very highly sulphated, are short; the length of the repeat region and chain caps is ca. nine disaccharides. These data demonstrate that the structure of the N-linked keratan sulphate chains of human articular cartilage fibromodulin is similar, in general, to articular cartilage derived O-linked keratan sulphate chains. Further, the general structure of the keratan sulphate chains attached to human articular cartilage fibromodulin has been found to be generally similar to that of both bovine and equine articular cartilage fibromodulin.

Item Type: Article
Journal or Publication Title: Glycoconjugate journal
Uncontrolled Keywords: fibromodulin ; small proteoglycan ; keratan sulphate ; glycosaminoglycan ; sulphation ; keratanase II ; human ; N-ACETYLNEURAMINIC ACID ; CARBOHYDRATE DIFFERENTIATION ANTIGENS ; BOVINE TRACHEAL CARTILAGE ; SULFATE CHAINS ; II DIGESTION ; COLLAGEN ; OLIGOSACCHARIDES ; PROTEOGLYCANS ; IDENTIFICATION ; CHROMATOGRAPHY
Subjects:
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 49065
Deposited By: ep_importer_pure
Deposited On: 18 Jul 2011 17:00
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 19:23
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/49065

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