Lancaster EPrints

FTIR Microspectroscopy Coupled with Two-Class Discrimination Segregates Markers Responsible for Inter- and Intra-Category Variance in Exfoliative Cervical Cytology.

Walsh, Michael J. and Singh, Maneesh N. and Stringfellow, Helen F. and Pollock, Hubert M. and Hammiche, Azzedine and Grude, Olaug and Fullwood, Nigel J. and Pitt, Mark A. and Martin-Hirsch, Pierre L. and Martin, Francis L. (2008) FTIR Microspectroscopy Coupled with Two-Class Discrimination Segregates Markers Responsible for Inter- and Intra-Category Variance in Exfoliative Cervical Cytology. Biomarker Insights, 3. pp. 179-189. ISSN 1177-2719

[img]
Preview
PDF (Biomarker_Paper.pdf)
Download (539Kb) | Preview

    Abstract

    Infrared (IR) absorbance of cellular biomolecules generates a vibrational spectrum, which can be exploited as a “biochemical fingerprint” of a particular cell type. Biomolecules absorb in the mid-IR (2–20 μm) and Fourier-transform infrared (FTIR) microspectroscopy applied to discriminate different cell types (exfoliative cervical cytology collected into buffered fixative solution) was evaluated. This consisted of cervical cytology free of atypia (i.e. normal; n = 60), specimens categorised as containing low-grade changes (i.e. CIN1 or LSIL; n = 60) and a further cohort designated as high-grade (CIN2/3 or HSIL; n = 60). IR spectral analysis was coupled with principal component analysis (PCA), with or without subsequent linear discriminant analysis (LDA), to determine if normal versus low-grade versus high-grade exfoliative cytology could be segregated. With increasing severity of atypia, decreases in absorbance intensity were observable throughout the 1,500 cm−1 to 1,100 cm−1 spectral region; this included proteins (1,460 cm−1), glycoproteins (1,380 cm−1), amide III (1,260 cm−1), asymmetric (νas) PO2 − (1,225 cm−1) and carbohydrates (1,155 cm−1). In contrast, symmetric (νs) PO2 − (1,080 cm−1) appeared to have an elevated intensity in high-grade cytology. Inter-category variance was associated with protein and DNA conformational changes whereas glycogen status strongly influenced intra-category. Multivariate data reduction of IR spectra using PCA with LDA maximises inter-category variance whilst reducing the influence of intra-class variation towards an objective approach to class cervical cytology based on a biochemical profile.

    Item Type: Article
    Journal or Publication Title: Biomarker Insights
    Uncontrolled Keywords: biomarker ; cervical cytology ; Fourier-transform infrared microspectroscopy ; high-grade ; low-grade ; principal component analysis
    Subjects: Q Science > QH Natural history > QH301 Biology
    Departments: Faculty of Science and Technology > Physics
    Faculty of Health and Medicine > Biomedical & Life Sciences
    Faculty of Science and Technology > Lancaster Environment Centre
    Faculty of Health and Medicine
    ID Code: 4515
    Deposited By: Mr Michael Walsh
    Deposited On: 27 Mar 2008 11:05
    Refereed?: Yes
    Published?: Published
    Last Modified: 06 Sep 2013 20:54
    Identification Number:
    URI: http://eprints.lancs.ac.uk/id/eprint/4515

    Actions (login required)

    View Item