Lancaster EPrints

Dynamic changes in functional gene copy numbers and microbial communities during degradation of pyrene in soils.

Peng, Jing-Jing and Cai, Chao and Qiao, Min and Li, Hong and Zhu, Yong-Guan (2010) Dynamic changes in functional gene copy numbers and microbial communities during degradation of pyrene in soils. Environmental Pollution, 158 (9). pp. 2872-2879. ISSN 0269-7491

Full text not available from this repository.

Abstract

This study investigates the dynamics of pyrene degradation rates, microbial communities, and functional gene copy numbers during the incubation of pyrene-spiked soils. Spiking pyrene to the soil was found to have negligible effects on the bacterial community present. Our results demonstrated that there was a signi fi cant difference in nidA gene copy numbers between sampling dates in QZ soil. Mycobacterium 16S rDNA clone libraries showed that more than 90% mycobacteria detected were closely related to fast- growing PAH-degrading Mycobacterium in pyrene-spiked soil, while other sequences related to slow-growing Mycobacterium were only detected in the control soil. It is suggested that nidA gene copy number and fast-growing PAH-degrading Mycobacterium could be used as indicators to predict pyrene contamination and its degradation activity in soils.

Item Type: Article
Journal or Publication Title: Environmental Pollution
Uncontrolled Keywords: nidA; qPCR; Mycobacterium; T-RFLP; Bacterial community
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
Q Science > QR Microbiology
Departments: Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 40807
Deposited By: Dr Hong Li
Deposited On: 23 May 2011 13:12
Refereed?: Yes
Published?: Published
Last Modified: 19 Sep 2012 11:11
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/40807

Actions (login required)

View Item