Peng, Jing-Jing and Cai, Chao and Qiao, Min and Li, Hong and Zhu, Yong-Guan (2010) Dynamic changes in functional gene copy numbers and microbial communities during degradation of pyrene in soils. Environmental Pollution, 158 (9). pp. 2872-2879. ISSN 0269-7491Full text not available from this repository.
This study investigates the dynamics of pyrene degradation rates, microbial communities, and functional gene copy numbers during the incubation of pyrene-spiked soils. Spiking pyrene to the soil was found to have negligible effects on the bacterial community present. Our results demonstrated that there was a signi ﬁ cant difference in nidA gene copy numbers between sampling dates in QZ soil. Mycobacterium 16S rDNA clone libraries showed that more than 90% mycobacteria detected were closely related to fast- growing PAH-degrading Mycobacterium in pyrene-spiked soil, while other sequences related to slow-growing Mycobacterium were only detected in the control soil. It is suggested that nidA gene copy number and fast-growing PAH-degrading Mycobacterium could be used as indicators to predict pyrene contamination and its degradation activity in soils.
|Journal or Publication Title:||Environmental Pollution|
|Uncontrolled Keywords:||nidA; qPCR; Mycobacterium; T-RFLP; Bacterial community|
|Subjects:||G Geography. Anthropology. Recreation > GE Environmental Sciences|
Q Science > QR Microbiology
|Departments:||Faculty of Science and Technology > Lancaster Environment Centre|
|Deposited By:||Dr Hong Li|
|Deposited On:||23 May 2011 13:12|
|Last Modified:||19 Sep 2012 11:11|
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