Peng, Jing-Jing and Cai, Chao and Qiao, Min and Li, Hong and Zhu, Yong-Guan (2010) Dynamic changes in functional gene copy numbers and microbial communities during degradation of pyrene in soils. Environmental Pollution, 158 (9). pp. 2872-2879. ISSN 0269-7491
Full text not available from this repository.Abstract
This study investigates the dynamics of pyrene degradation rates, microbial communities, and functional gene copy numbers during the incubation of pyrene-spiked soils. Spiking pyrene to the soil was found to have negligible effects on the bacterial community present. Our results demonstrated that there was a signi fi cant difference in nidA gene copy numbers between sampling dates in QZ soil. Mycobacterium 16S rDNA clone libraries showed that more than 90% mycobacteria detected were closely related to fast- growing PAH-degrading Mycobacterium in pyrene-spiked soil, while other sequences related to slow-growing Mycobacterium were only detected in the control soil. It is suggested that nidA gene copy number and fast-growing PAH-degrading Mycobacterium could be used as indicators to predict pyrene contamination and its degradation activity in soils.
| Item Type: | Article |
|---|---|
| Journal or Publication Title: | Environmental Pollution |
| Uncontrolled Keywords: | nidA; qPCR; Mycobacterium; T-RFLP; Bacterial community |
| Subjects: | G Geography. Anthropology. Recreation > GE Environmental Sciences Q Science > QR Microbiology |
| Departments: | Faculty of Science and Technology > Lancaster Environment Centre |
| ID Code: | 40807 |
| Deposited By: | Dr Hong Li |
| Deposited On: | 23 May 2011 13:12 |
| Refereed?: | Yes |
| Published?: | Published |
| Last Modified: | 19 Sep 2012 11:11 |
| Identification Number: | |
| URI: | http://eprints.lancs.ac.uk/id/eprint/40807 |
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