Lancaster EPrints

Cadmium and copper inhibit both DNA repair activities of polynucleotide kinase.

Whiteside, James R. and Box, Clare L. and McMillan, Trevor J. and Allinson, Sarah L. (2010) Cadmium and copper inhibit both DNA repair activities of polynucleotide kinase. DNA Repair, 9 (1). pp. 83-89. ISSN 1568-7864

Full text not available from this repository.

Abstract

Human exposure to heavy metals is of increasing concern due to their well-documented toxicological and carcinogenic effects and rising environmental levels through industrial processes and pollution. It has been widely reported that such metals can be genotoxic by several modes of action including generation of reactive oxygen species and inhibition of DNA repair. However, although it has been observed that certain heavy metals can inhibit single strand break (SSB) rejoining, the effects of these metals on SSB end-processing enzymes has not previously been investigated. Accordingly, we have investigated the potential inhibition of polynucleotide kinase (PNK)-dependent single strand break repair by six metals: cadmium, cobalt, copper, nickel, lead and zinc. It was found that micromolar concentrations of cadmium and copper are able to inhibit the phosphatase and kinase activities of PNK in both human cell extracts and purified recombinant protein, while the other metals had no effect at the concentrations tested. The inhibition of PNK by environmentally and physiologically relevant concentrations of cadmium and copper suggests a novel means by which these toxic heavy metals may exert their carcinogenic and neurotoxic effects.

Item Type: Article
Journal or Publication Title: DNA Repair
Uncontrolled Keywords: Heavy metals ; Polynucleotide kinase ; Single strand breaks ; Cadmium ; Copper
Subjects: R Medicine > RL Dermatology
Departments:
ID Code: 40738
Deposited By: Professor Trevor McMillan
Deposited On: 28 Apr 2011 09:07
Refereed?: Yes
Published?: Published
Last Modified: 23 Jul 2014 14:52
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/40738

Actions (login required)

View Item