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A bystander effect is induced in human cells treated with UVA radiation but not UVB radiation.

Whiteside, James R. and McMillan, Trevor J. (2009) A bystander effect is induced in human cells treated with UVA radiation but not UVB radiation. Radiation Research, 171 (2). pp. 204-211. ISSN 0033-7587

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Abstract

The bystander effect is defined as the induction of damage in nonirradiated cells by irradiated cells. The significance on health is unknown, but the effective amplification of deleterious effects of cytotoxic agents is a concern. In this study the ability of UVA or UVB radiation to induce the bystander effect in human keratinocytes (HaCaT) and fibroblasts (MRC5) was examined using a method that involved the co-incubation of two differentially treated cell populations separated by a medium-permeable insert. This allowed the study of interactions between cells in the absence of direct cell-to-cell contact. Irradiation of one population with 100 kJ/m(2) UVA radiation induced a bystander effect in a second population of unirradiated cells that was manifested as reduced clonogenic survival. This effect was induced within and between the two cell lines but was not seen after treatment with 400 J/m(2) UVB radiation. An additional reduction in survival above that expected to occur as a result of direct exposure was observed when the two UVA-irradiated populations were co-incubated. As well as providing some potentially important information regarding the biological effects of UV light, the spectral variation in the induction of the effect provides a useful approach to dissecting the mechanisms underlying such effects

Item Type: Article
Journal or Publication Title: Radiation Research
Subjects: R Medicine > RL Dermatology
Departments: Faculty of Science and Technology > Lancaster Environment Centre
VC's Office
ID Code: 40736
Deposited By: Professor Trevor McMillan
Deposited On: 28 Apr 2011 08:58
Refereed?: Yes
Published?: Published
Last Modified: 09 Apr 2014 22:04
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/40736

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