Lancaster EPrints

Cell morphogenesis of Trypanosoma brucei requires the paralogous, differentially expressed calpain-related proteins CAP5.5 and CAP5.5V.

Olego-Fernandez, Sofia and Vaughan, Sue and Shaw, Michael K. and Gull, Keith and Ginger, Michael L. (2009) Cell morphogenesis of Trypanosoma brucei requires the paralogous, differentially expressed calpain-related proteins CAP5.5 and CAP5.5V. Protist, 160 (4). pp. 576-590. ISSN 1434-4610

Full text not available from this repository.

Abstract

Proteins from the calpain super-family are involved in developmentally- and environmentally-regulated re-modelling of the eukaryotic cytoskeleton and the dynamic organisation of signal transduction cascades. In trypanosomatid parasites, calpain-related gene families are unusually large, but we have little insight into the functional roles played by these molecules during trypanosomatid lifecycles. Here we report that CAP5.5, a cytoskeletal calpain-related protein subject to strict stage-specific expression in the sleeping sickness parasite Trypanosoma brucei, is essential and required for correct cell morphogenesis of procyclic (tsetse mid-gut stage) T. brucei. Striking consequences of CAP5.5 RNA interference are the loss of protein from the posterior cell-end, organelle mis-positioning giving rise to aberrant cytokinesis, and disorganisation of the sub-pellicular microtubules that define trypanosome cell shape. We further report that the stage-specificity of CAP5.5 expression can be explained by the presence of a paralogue, CAP5.5V, which is required for cell morphogenesis in bloodstream T. brucei; RNAi against this paralogous protein results in a qualitatively similar phenotype to that described for procyclic CAP5.5 RNAi mutants. By comparison to recently described phenotypes for other procyclic trypanosome RNAi mutants, likely functions for CAP5.5 and CAP5.5V are discussed.

Item Type: Article
Journal or Publication Title: Protist
Uncontrolled Keywords: calpain ; cytoskeleton ; microtubule ; procyclic ; trypomastigote ; trypanosome
Subjects: Q Science > QH Natural history > QH301 Biology
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 34895
Deposited By: Dr Michael Ginger
Deposited On: 14 Dec 2010 14:27
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 17:43
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/34895

Actions (login required)

View Item