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Syrian hamster embryo (SHE) assay (pH 6.7) coupled with infrared spectroscopy and chemometrics towards toxicological assessment

Trevisan, Julio and Angelov, Plamen P. and Patel, Imran I. and Najand, Ghazel M. and Cheung, Karen T. and Llabjani, Valon and Pollock, Hubert M. and Bruce, Shannon W. and Pant, Kamala and Carmichael, Paul M. and Scott, Andrew D. and Martin, Francis L. (2010) Syrian hamster embryo (SHE) assay (pH 6.7) coupled with infrared spectroscopy and chemometrics towards toxicological assessment. Analyst, 135 (12). pp. 3266-3272. ISSN 0003-2654

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Abstract

The Syrian hamster embryo (SHE) assay (pH 6.7) is an in vitro candidate to replace in vivo carcinogenicity tests. However, the conventional method of visual scoring of foci (non-transformed vs. transformed colonies) can be time-consuming and is open to subjectivity. Infrared (IR) spectroscopy has the potential to provide objective assessment of such SHE colonies with the added advantage of potentially providing mechanistic information. In this study, SHE cells were treated with one of eight different chemical regimens, allowed in culture to attach and form foci on IR-reflective glass slides; these were subsequently interrogated by attenuated total reflection (ATR) Fourier-transform IR (FTIR) spectroscopy. Derived mid-IR spectra (n=13,406) were subjected to chemometric analysis focusing primarily on the extraction of biochemical information related to test agent treatment and/or morphological transformation. The use of ATR-FTIR spectroscopy with chemometrics to analyze the SHE assay is a novel approach to toxicological assessment.

Item Type: Article
Journal or Publication Title: Analyst
Subjects: Q Science > QA Mathematics > QA75 Electronic computers. Computer science
Q Science > QH Natural history > QH301 Biology
Q Science > QC Physics
Departments: UNSPECIFIED
ID Code: 34264
Deposited By: Dr H M Pollock
Deposited On: 24 Sep 2010 09:10
Refereed?: Yes
Published?: Published
Last Modified: 23 Jul 2014 14:51
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/34264

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