Mustacchi, Roberta and Knowles, Christopher J. and Li, Hong and Dalrymple, Ian and Sunderland, Garry and Skibar, Wolfgang and Jackman, Simon A. (2005) Enhanced biotransformations and product recovery in a membrane bioreactor through application of a direct electric current. Biotechnology and Bioengineering, 89 (1). pp. 18-23. ISSN 1097-0290Full text not available from this repository.
The simultaneous enhancement of biotransformation coupled to product recovery, purification and concentration is presented. The nitrilase of Rhodococcus rhodochrous LL100-21 catalyses the single-step hydrolytic biotransformation of benzonitrile to benzoic acid and ammonia. When a direct electric current is applied across a bioreactor containing the bacterium and benzonitrile, the charged product (benzoic acid) can be removed in situ across an anion exchange membrane and recovered in a separate compartment. Over the course of a 24-hour biotransformation, benzonitrile was converted to benzoic acid which was completely removed from the bioreactor chamber and concentrated 3-fold in a separate chamber. The rate of production of benzoic acid increased by 42% when the current was applied (0.044 mmol/min/g dry cell weight in the presence of current as compared to 0.03 mmol/min/g dry cell weight in its absence). The enhanced reaction rate was achieved irrespective of product separation and therefore appears to be a direct effect upon the bacterial cells. This process has potential for enhanced productivity from biotransformations through a simultaneous increase in metabolic activity and in situ product recovery.
|Journal or Publication Title:||Biotechnology and Bioengineering|
|Additional Information:||Funded by: the United Kingdom Department of Trade and Industry and the Biotechnology and Biological Sciences Research Council|
|Uncontrolled Keywords:||electrokinetics • direct current • nitriles • biotransformations • in situ product recovery|
|Subjects:||G Geography. Anthropology. Recreation > GE Environmental Sciences|
|Departments:||Faculty of Science and Technology > Lancaster Environment Centre|
|Deposited By:||Mrs Yaling Zhang|
|Deposited On:||28 Jan 2010 10:05|
|Last Modified:||02 Mar 2016 01:10|
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