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Enhanced biotransformations and product recovery in a membrane bioreactor through application of a direct electric current.

Mustacchi, Roberta and Knowles, Christopher J. and Li, Hong and Dalrymple, Ian and Sunderland, Garry and Skibar, Wolfgang and Jackman, Simon A. (2005) Enhanced biotransformations and product recovery in a membrane bioreactor through application of a direct electric current. Biotechnology and Bioengineering, 89 (1). pp. 18-23. ISSN 1097-0290

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Abstract

The simultaneous enhancement of biotransformation coupled to product recovery, purification and concentration is presented. The nitrilase of Rhodococcus rhodochrous LL100-21 catalyses the single-step hydrolytic biotransformation of benzonitrile to benzoic acid and ammonia. When a direct electric current is applied across a bioreactor containing the bacterium and benzonitrile, the charged product (benzoic acid) can be removed in situ across an anion exchange membrane and recovered in a separate compartment. Over the course of a 24-hour biotransformation, benzonitrile was converted to benzoic acid which was completely removed from the bioreactor chamber and concentrated 3-fold in a separate chamber. The rate of production of benzoic acid increased by 42% when the current was applied (0.044 mmol/min/g dry cell weight in the presence of current as compared to 0.03 mmol/min/g dry cell weight in its absence). The enhanced reaction rate was achieved irrespective of product separation and therefore appears to be a direct effect upon the bacterial cells. This process has potential for enhanced productivity from biotransformations through a simultaneous increase in metabolic activity and in situ product recovery.

Item Type: Article
Journal or Publication Title: Biotechnology and Bioengineering
Additional Information: Funded by: the United Kingdom Department of Trade and Industry and the Biotechnology and Biological Sciences Research Council
Uncontrolled Keywords: electrokinetics • direct current • nitriles • biotransformations • in situ product recovery
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
Departments: Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 31618
Deposited By: Mrs Yaling Zhang
Deposited On: 28 Jan 2010 10:05
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 16:59
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/31618

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