Mustacchi, Roberta and Knowles, Christopher J. and Li, Hong and Dalrymple, Ian and Sunderland, Garry and Skibar, Wolfgang and Jackman, Simon A. (2005) Enhanced biotransformations and product recovery in a membrane bioreactor through application of a direct electric current. Biotechnology and Bioengineering, 89 (1). pp. 18-23. ISSN 1097-0290
Full text not available from this repository.Abstract
The simultaneous enhancement of biotransformation coupled to product recovery, purification and concentration is presented. The nitrilase of Rhodococcus rhodochrous LL100-21 catalyses the single-step hydrolytic biotransformation of benzonitrile to benzoic acid and ammonia. When a direct electric current is applied across a bioreactor containing the bacterium and benzonitrile, the charged product (benzoic acid) can be removed in situ across an anion exchange membrane and recovered in a separate compartment. Over the course of a 24-hour biotransformation, benzonitrile was converted to benzoic acid which was completely removed from the bioreactor chamber and concentrated 3-fold in a separate chamber. The rate of production of benzoic acid increased by 42% when the current was applied (0.044 mmol/min/g dry cell weight in the presence of current as compared to 0.03 mmol/min/g dry cell weight in its absence). The enhanced reaction rate was achieved irrespective of product separation and therefore appears to be a direct effect upon the bacterial cells. This process has potential for enhanced productivity from biotransformations through a simultaneous increase in metabolic activity and in situ product recovery.
| Item Type: | Article |
|---|---|
| Journal or Publication Title: | Biotechnology and Bioengineering |
| Additional Information: | Funded by: the United Kingdom Department of Trade and Industry and the Biotechnology and Biological Sciences Research Council |
| Uncontrolled Keywords: | electrokinetics • direct current • nitriles • biotransformations • in situ product recovery |
| Subjects: | G Geography. Anthropology. Recreation > GE Environmental Sciences |
| Departments: | Faculty of Science and Technology > Lancaster Environment Centre |
| ID Code: | 31618 |
| Deposited By: | Mrs Yaling Zhang |
| Deposited On: | 28 Jan 2010 10:05 |
| Refereed?: | Yes |
| Published?: | Published |
| Last Modified: | 26 Jul 2012 16:59 |
| Identification Number: | |
| URI: | http://eprints.lancs.ac.uk/id/eprint/31618 |
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