Zhao, Bojun and Ma, Aihua and Martin, Frank L. and Fullwood, Nigel J. (2009) An investigation into corneal alkali burns using an organ culture model. Cornea, 28 (5). pp. 541-546. ISSN 0277-3740Full text not available from this repository.
Purpose: To evaluate the usefulness of an in vitro corneal organ culture system for Studying severe alkali hunts. Methods: Fresh bovine cornea was cultured using an established organ culture system. Two molar sodium hydroxide was applied to the corneal and limbal epithelia for 60 seconds. Gross and ultrastructural changes were evaluated at different time points over a 1-week period. Results: The condition of the alkali-burned cornea deteriorated in a time-dependent manner over the 1-week period. Gross changes were evident immediately, and ultrastructural changes were detected in the epithelium, stroma, and endothelium at 1 hour after the alkali burn. By 7 days, most of the corneal and limbal epithelia were destroyed. The corneal stroma was disrupted with separation of lamella and fragmentation of collagen fibrils. By day 7, the endothelium was reduced to cellular debris, although Descemet membrane remained intact. Conclusions: The changes observed in the in vitro organ culture model in response to a severe alkali burn are similar to those observed by other groups in clinical and in vivo studies. We believe that this or similar organ culture systems could be used to evaluate some aspects of severe alkali burns.
|Journal or Publication Title:||Cornea|
|Uncontrolled Keywords:||corneal organ culture ; alkali burns ; stem cells ; electron microscopy ; AMNIOTIC MEMBRANE ; RABBIT CORNEA ; CELLS ; TRANSPLANTATION ; EPITHELIUM ; MICROSPECTROSCOPY ; ULTRASTRUCTURE|
|Subjects:||G Geography. Anthropology. Recreation > GE Environmental Sciences|
|Departments:||Faculty of Health and Medicine > Biomedical & Life Sciences|
Faculty of Science and Technology > Lancaster Environment Centre
|Deposited By:||Mr Richard Ingham|
|Deposited On:||13 Jan 2010 13:16|
|Last Modified:||21 Mar 2013 14:58|
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