Walsh, Michael John and Hammiche, Azzedine and Fellous, Tariq G. and Nicholson, James M. and Cotte, Marine and Susini, Jean and Fullwood, Nigel J. and Martin-Hirsch, Pierre L. and Alison, Malcolm R. and Martin, Frank L. (2009) Tracking the cell hierarchy in the human intestine using biochemical signatures derived by mid-infrared microspectroscopy. Stem Cell Research, 3 (1). pp. 15-27. ISSN 1873-5061
Full text not available from this repository.Abstract
Markers of gastrointestinal (GI) stem cells remain elusive. We employed synchrotron Fourier-transform infrared (FTIR) microspectroscopy to derive mid-infrared (IR) spectra along the length of human GI crypts. Tissue sections (10-μm thick) were floated onto BaF2 windows and image maps were acquired of small intestine and large bowel crypts in transmission mode with an aperture of ≤ 10 μm × 10 μm. Counting upwards in a step-size (≤ 10 μm) fashion from the crypt base, IR spectra were extracted from the image maps and each spectrum corresponding to a particular location was identified. Spectra were analyzed using principal component analysis plus linear discriminant analysis. Compared to putative crypt base columnar/Paneth cells, those assigned as label-retaining cells were chemically more similar to putative large bowel stem cells and, the small intestine transit-amplifying cells were closest to large bowel transit-amplifying cells; interestingly, the base of small intestine crypts was the most chemically-distinct. This study suggests that in the complex cell lineage of human GI crypts, chemical similarities as revealed by FTIR microspectroscopy between regions putatively assigned as stem cell, transit-amplifying and terminally-differentiated facilitates identification of cell function.
| Item Type: | Article |
|---|---|
| Journal or Publication Title: | Stem Cell Research |
| Subjects: | G Geography. Anthropology. Recreation > GE Environmental Sciences Q Science > QC Physics |
| Departments: | Faculty of Science and Technology > Lancaster Environment Centre Faculty of Science and Technology > Physics Faculty of Health and Medicine > Biomedical & Life Sciences Faculty of Health and Medicine |
| ID Code: | 31356 |
| Deposited By: | Mr Richard Ingham |
| Deposited On: | 13 Jan 2010 11:12 |
| Refereed?: | Yes |
| Published?: | Published |
| Last Modified: | 26 Jul 2012 16:56 |
| Identification Number: | |
| URI: | http://eprints.lancs.ac.uk/id/eprint/31356 |
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