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Application of a luminescence-based biosensor for assessing naphthalene biodegradation in soils from a manufactured gas plant.

Paton, Graeme I. and Reid, Brian J. and Semple, Kirk T. (2009) Application of a luminescence-based biosensor for assessing naphthalene biodegradation in soils from a manufactured gas plant. Environmental Pollution, 157 (5). pp. 1643-1648. ISSN 0269-7491

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Abstract

Despite numerous reviews suggesting that microbial biosensors could be used in many environmental applications, in reality they have failed to be used for which they were designed. In part this is because most of these sensors perform in an aqueous phase and a buffered medium, which is in contrast to the nature of genuine environmental systems. In this study, a range of non-exhaustive extraction techniques (NEETs) were assessed for (i) compatibility with a naphthalene responsive biosensor and (ii) correlation with naphthalene biodegradation. The NEETs removed a portion of the total soil naphthalene in the order of methanol > HPCD > βCD > water. To place the biosensor performance to NEETs in context, a biodegradation experiment was carried out using historically contaminated soils. By coupling the HPCD extraction with the biosensor, it was possible to assess the fraction of the naphthalene capable of undergoing microbial degradation in soil. Exposure of microbial biosensors to cyclodextrin solutions allows the assessment of the degradable fraction of contaminants in soil.

Item Type: Article
Journal or Publication Title: Environmental Pollution
Uncontrolled Keywords: Bioluminescent bacteria ; Organic contaminants ; Biodegradation ; Extraction ; Bioavailability
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
Departments: Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 30730
Deposited By: Mr Richard Ingham
Deposited On: 02 Dec 2009 14:45
Refereed?: Yes
Published?: Published
Last Modified: 06 Sep 2013 19:41
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/30730

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