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The Mre11/Rad50/Nbs1 complex functions in resection-based DNA end joining in Xenopus laevis.

Taylor, Elaine M. and Cecillon, Sophie M. and Bonis, Antonio and Chapman, J. Ross and Povirk, Lawrence F. and Lindsay, Howard D. (2010) The Mre11/Rad50/Nbs1 complex functions in resection-based DNA end joining in Xenopus laevis. Nucleic Acids Research, 38 (2). pp. 441-454. ISSN 0305-1048

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Abstract

The repair of DNA double-strand breaks (DSBs) is essential to maintain genomic integrity. In higher eukaryotes, DNA DSBs are predominantly repaired by non-homologous end joining (NHEJ), but DNA ends can also be joined by an alternative error-prone mechanism termed microhomologymediated end joining (MMEJ). In MMEJ, the repair of DNA breaks is mediated by annealing at regions of microhomology and is always associated with deletions at the break site. In budding yeast, the Mre11/Rad5/Xrs2 complex has been demonstrated to play a role in both classical NHEJ and MMEJ, but the involvement of the analogous MRE11/RAD50/ NBS1 (MRN) complex in end joining in higher eukaryotes is less certain. Here we demonstrate that in Xenopus laevis egg extracts, the MRN complex is not required for classical DNA-PKdependent NHEJ. However, the XMRN complex is necessary for resection-based end joining of mismatched DNA ends. This XMRN-dependent end joining process is independent of the core NHEJ components Ku70 and DNA-PK, occurs with delayed kinetics relative to classical NHEJ and brings about repair at sites of microhomology. These data indicate a role for the X. laevis MRN complex in MMEJ.

Item Type: Article
Journal or Publication Title: Nucleic Acids Research
Subjects: Q Science > QH Natural history > QH301 Biology
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
Faculty of Health and Medicine > Medicine
ID Code: 28224
Deposited By: Dr Howard Lindsay
Deposited On: 17 Nov 2009 13:16
Refereed?: Yes
Published?: Published
Last Modified: 01 Oct 2013 11:21
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/28224

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