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FTIR micro-spectroscopy identifies symmetric PO2- modifications as a marker of the putative stem cell region of human intestinal crypts.

Walsh, Michael J. and Fellous, Tariq G. and Hammiche, Azzedine and Lin, Wey-Ran and Fullwood, Nigel J. and Grude, Olaug and Bahrami, Fariba and Nicholson, James M. and Cotte, Marine and Susini, Jean and Pollock, Hubert M. and Brittan, Mairi and Martin-Hirsch, Pierre L. and Alison, Malcolm R. and Martin, Francis L. (2008) FTIR micro-spectroscopy identifies symmetric PO2- modifications as a marker of the putative stem cell region of human intestinal crypts. Stem Cells, 26 (10). pp. 108-118. ISSN 1549-4918

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Abstract

Complex biomolecules absorb in the mid-infrared ( = 2-20 m) giving vibrational spectra associated with structure and function. We employed Fourier transform infrared (FTIR) micro-spectroscopy to “fingerprint” locations along the length of human small and large intestinal crypts. Paraffin-embedded slices of normal human gut were sectioned (10-m thick) and mounted to facilitate infrared (IR) spectral analyses. IR spectra were collected employing globar (15 m  15 m aperture) FTIR micro-spectroscopy in reflection mode, synchrotron (10 m  10 m aperture) FTIR micro-spectroscopy in transmission mode, or near-field photothermal micro-spectroscopy (PTMS). Dependent on the location of crypt interrogation, clear differences in spectral characteristics were noted. Epithelial-cell IR spectra were subjected to principal component analysis to determine whether wavenumber-absorbance relationships expressed as single points in “hyperspace” might on the basis of multivariate distance reveal biophysical differences between cells in situ along the length of gut crypts. Following spectroscopic analysis, plotted clusters and their loadings plots pointed towards symmetric (s) PO2- (1080 cm-1) vibrations as a discriminating factor for the putative stem cell region of crypts. This was subsequently confirmed by image mapping and points to a novel approach of deriving an integrated biochemical fingerprint of a tissue’s stem cell niche and identifying its spatial location in a non-destructive fashion. These results suggest that DNA conformational alterations associated with cells residing in the putative stem cell region of crypts can be used as a means of identification, which may have utility in other tissues where the location of the niche is unclear.

Item Type: Article
Journal or Publication Title: Stem Cells
Uncontrolled Keywords: Crypts ; FTIR micro-spectroscopy ; Intestinal ; Principal component analysis ; Symmetric phosphate ; Synchrotron
Subjects: Q Science > QC Physics
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
Faculty of Science and Technology > Physics
Faculty of Science and Technology > Lancaster Environment Centre
Faculty of Health and Medicine
ID Code: 26724
Deposited By: Dr H M Pollock
Deposited On: 06 Jul 2009 10:22
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 16:35
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/26724

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