Lancaster EPrints

Isoprene synthesis protects transgenic tobacco plants from oxidative stress

Vickers, Claudia E. and Possell, Malcolm and Cojocariu, Christian and Velikova, Violeta B. and Laothawornkitkul, Jullada and Ryan, Annette and Mullineaux, Philip M. and Hewitt, C. N. (2009) Isoprene synthesis protects transgenic tobacco plants from oxidative stress. Plant, Cell and Environment, 32 (5). pp. 520-531. ISSN 0140-7791

Full text not available from this repository.

Abstract

Isoprene emission represents a significant loss of carbon to those plant species that synthesize this highly volatile and reactive compound. As a tool for studying the role of isoprene in plant physiology and biochemistry, we developed transgenic tobacco plants capable of emitting isoprene in a similar manner to and at rates comparable to a naturally emitting species. Thermotolerance of photosynthesis against transient high-temperature episodes could only be observed in lines emitting high levels of isoprene; the effect was very mild and could only be identified over repetitive stress events. However, isoprene-emitting plants were highly resistant to ozone-induced oxidative damage compared with their non-emitting azygous controls. In ozone-treated plants, accumulation of toxic reactive oxygen species (ROS) was inhibited, and antioxidant levels were higher. Isoprene-emitting plants showed remarkably decreased foliar damage and higher rates of photosynthesis compared to non-emitting plants immediately following oxidative stress events. An inhibition of hydrogen peroxide accumulation in isoprene-emitting plants may stall the programmed cell death response which would otherwise lead to foliar necrosis. These results demonstrate that endogenously produced isoprene provides protection from oxidative damage.

Item Type: Article
Journal or Publication Title: Plant, Cell and Environment
Uncontrolled Keywords: isoprene synthase ; oxidative damage ; ozone ; reactive oxygen species ; thermotolerance ; REACTION-MASS-SPECTROMETRY ; VOLATILE ORGANIC-COMPOUNDS ; PTR-MS ; INCREASES THERMOTOLERANCE ; NEGATIVE CONSEQUENCES ; LIPID-PEROXIDATION ; COMPOUND EMISSIONS ; HYDROGEN-PEROXIDE ; LEAVES ; OZONE
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
Departments: Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 26581
Deposited By: Prof CN Hewitt
Deposited On: 05 Jun 2009 15:09
Refereed?: Yes
Published?: Published
Last Modified: 06 Sep 2013 19:19
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/26581

Actions (login required)

View Item