Pritchard, D. I. and McKean, Paul G. and Rogan, M. T. and Schad, G. A. (1990) The identification of a species-specific antigen from Necator americanus. Parasite Immunology, 12 (3). pp. 259-267.Full text not available from this repository.
A 17 kD protein of Necator americanus was isolated by SDS-PAGE and used to raise monospecific antisera in rabbits. ELISA and Western blotting against a range of parasite extracts demonstrated the species specificity of this protein. It is expressed at all stages of the life-cycle, appears to be accumulated through the larval stages to adulthood, and can be localized in the oesophageal glands and cuticle of the adult parasite. The possible nature and diagnostic potential of this protein is discussed.
|Journal or Publication Title:||Parasite Immunology|
|Uncontrolled Keywords:||hookworm • diagnosis • antigen • ELISA • Western blotting • SDS-PAGE • colloidal gold • electron microscopy|
|Subjects:||Q Science > QH Natural history > QH301 Biology|
|Departments:||Faculty of Health and Medicine > Biomedical & Life Sciences|
|Deposited By:||Dr Paul G McKean|
|Deposited On:||12 Mar 2009 10:22|
|Last Modified:||07 Jan 2015 13:56|
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