McKean, Paul G. and O’Dea, Keiran P. and Brown, K. Neil (1993) Nucleotide sequence analysis and epitope mapping of the merozoite surface protein 1 from Plasmodium chabaudi chabaudi AS. Molecular and Biochemical Parasitology, 62 (2). pp. 199-209. ISSN 0166-6851Full text not available from this repository.
The complete nucleotide sequence of the gene encoding the merozoite surface protein 1 (MSP-1) from the rodent malaria parasite Plasmodium chabaudi chabaudi AS has been determined by direct sequencing of overlapping PCR derived fragments. Comparison of the P. c. chabaudi AS nucleotide sequence with the previously published P. c. chabaudi IP-PC1 sequence indicates that although the MSP-1 gene of these two P. c. chabaudi strains is highly conserved, with sequence identity often approaching 100%, interspersed throughout the molecule are 5 regions of divergence. This is at variance with published data which suggested that the P. c. chabaudi AS and P. c. chabaudi IP-PC1 MSP-1 sequences are largely identical. Epitope mapping studies with a panel of anti-P. c. chabaudi AS MSP-1 monoclonal antibodies demonstrate that whilst most of these mAbs recognise epitopes at the N-terminus of the MSP-1 molecule, two mAbs, including one capable of inhibiting challenge infections in mice in an in vivo passive transfer assay, recognise epitopes which map to the C-terminus.
|Journal or Publication Title:||Molecular and Biochemical Parasitology|
|Uncontrolled Keywords:||Malaria ; Plasmodium chabaudi chabaudi ; Merozoite surface protein 1 ; Nucleotide sequence analysis ; Epitope mapping|
|Subjects:||Q Science > QH Natural history > QH301 Biology|
|Departments:||Faculty of Health and Medicine > Biomedical & Life Sciences|
Faculty of Arts & Social Sciences > Linguistics & English Language
|Deposited By:||Dr Paul G McKean|
|Deposited On:||12 Mar 2009 10:13|
|Last Modified:||26 Jul 2012 16:28|
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