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A single amino acid determines the specificity of a monoclonal antibody which inhibits Plasmodium chabaudi AS in vivo.

McKean, Paul G. and O'Dea, Keiran P. and Brown, K. Neil (1993) A single amino acid determines the specificity of a monoclonal antibody which inhibits Plasmodium chabaudi AS in vivo. Molecular and Biochemical Parasitology, 62 (2). pp. 211-221. ISSN 0166-6851

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Abstract

The in vivo inhibitory action of NIMP23, a monoclonal antibody raised against the rodent parasite Plasmodium chabaudi chabaudi AS, has previously been shown to be strain-specific, capable of delaying significantly the onset of P. c. chabaudi AS but not a P. c. chabaudi CB challenge parasitaemia. The epitope to which this mAb binds has been mapped to the second of two epidermal growth factor-like domains located at the C-terminus of the merozoite surface protein 1 (MSP-1) of P. c. chabaudi AS. The C-terminus region of the MSP-1 of P. c. chabaudi is a region of heterogeneity with AS and CB strain parasites showing only 78% identity at the amino acid level. The critical amino acid substitution which accounts for the strain specificity of the NIMP23 monoclonal antibody has now been identified. Polymerase chain reaction directed mutagenesis experiments demonstrate that a single proline to asparagine substitution at position 1722 in the primary amino acid sequence is sufficient to convert NIMP23-negative P. c. chabaudi CB expression constructs into NIMP23-positive clones whilst the converse substitution of an asparagine for a proline residue converts P. c. chabaudi AS expression constructs into NIMP23-negative clones.

Item Type: Article
Journal or Publication Title: Molecular and Biochemical Parasitology
Uncontrolled Keywords: Malaria ; Plasmodium chabaudi chabaudi ; Merozoite surface protein 1 ; Epitope mapping ; Epidermal growth factor ; Antigenic diversity
Subjects: Q Science > QH Natural history > QH301 Biology
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
Faculty of Arts & Social Sciences > Linguistics & English Language
ID Code: 26055
Deposited By: Dr Paul G McKean
Deposited On: 12 Mar 2009 10:06
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 16:28
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/26055

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