McKean, Paul G. and Delahay, Rob and Pimenta, Paulo F. and Smith, Deborah F. (1997) Characterisation of a second protein encoded by the differentially regulated LmcDNA16 gene family of Leishmania major. Molecular and Biochemical Parasitology, 85 (2). pp. 221-231. ISSN 0166-6851
Full text not available from this repository.Abstract
The LmcDNA16 gene family of Leishmania major contains five genes: three highly related sequences, genes A, B and C, and a tandem pair of unrelated sequences, genes D1 and D2. Previous studies have demonstrated that gene B codes for a novel, hydrophilic protein that is present on the surface of infective parasite stages at approximately 10(5) copies per call. This paper describes the identification and characterisation of a second protein encoded by this gene array: the 7.6 kDa A/C protein. This molecule shares considerable amino acid identity with the gene B protein (GBP) but lacks the characteristic proline rich amino acid repeat region. Like GBP, the A/C protein is expressed on the surface of infective metacyclic parasites, despite the lack of conventional signal and anchor sequences. It has previously been suggested that the GBP repetitive sequence plays a role in mediating protein attachment to the parasite surface. It now appears more likely that the conserved amino- and/or carboxyl-terminal domains of the A/C and B proteins are involved in this process.
| Item Type: | Article |
|---|---|
| Journal or Publication Title: | Molecular and Biochemical Parasitology |
| Uncontrolled Keywords: | Leishmania major ; Gene family ; Surface proteins ; Amino acid repeats |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Departments: | Faculty of Health and Medicine > Biomedical & Life Sciences |
| ID Code: | 26051 |
| Deposited By: | Dr Paul G McKean |
| Deposited On: | 12 Mar 2009 09:44 |
| Refereed?: | Yes |
| Published?: | Published |
| Last Modified: | 26 Jul 2012 16:28 |
| Identification Number: | |
| URI: | http://eprints.lancs.ac.uk/id/eprint/26051 |
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