Huddart, H. and Nelson, I. D. and Brooks, D. D. and Hill, R. B. (1992) The calcium dependence of electrical and mechanical responses of the odontophore protractor muscle of Busycon canaliculatum. A sucrose-gap study of calcium antagonist action. Comparative Biochemistry and Physiology Part A: Physiology, 102 (2). pp. 299-305.Full text not available from this repository.
1. 1. Exposures for 30 min to Ca-free salines irreversibly inhibited responses of the odontophore protractor (OP) muscles of Busycon canaliculatum to ACh and high K+ salines. Under continuous field stimulation, Ca-free salines extinguished the responses within 3 min and recovery on Ca readmission was only partial. 2. 2. Nifedipine converted normally smooth ACh-induced depolarizations into oscillatory events generating small twitch-like contractions. 3. 3. Nifedipine converted normally tonic high K+ responses into depolarizations with fast spike-like action potentials generating fast twitches. The low K+induced action potentials and fast twitches of this muscle were greatly enhanced by nifedipine. Nifedipine induced rapid transient inward current pulses accompanying this twitch activity. 4. 4. Co2+, Cd2+ and Gd3+ all inhibited the ACh and K+ responses of the OP muscle but Gd3+ was the most consistent and potent inhibitor of these responses. 5. 5. Gd3+ did not affect K+- or ACh-induced depolarization levels but eliminated spike-like action potentials and the twitches they generated. 6. 6. When the muscle membranes were depolarized with increasing K+ subsequent ACh responses were inhibited and eventually eliminated at about −41 mV, indicating that the ACh receptor here showed voltage inactivation. 7. 7. Although dependent upon an influx of[Ca]0 for CIRC to effect EC coupling, this Ca appears to enter the cells by a mechanism unlike that of mammalian smooth muscle. The cellular Ca pool appears to be independently but not synergistically accessed by ACh and K+ in the induction of contractile responses.
|Journal or Publication Title:||Comparative Biochemistry and Physiology Part A: Physiology|
|Subjects:||G Geography. Anthropology. Recreation > GE Environmental Sciences|
|Departments:||Faculty of Arts & Social Sciences > Politics & International Relations (Merged into PPR 2010-08-01)|
Faculty of Science and Technology > Lancaster Environment Centre
|Deposited On:||09 Jan 2009 11:24|
|Last Modified:||07 Jan 2015 13:43|
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