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Degradation of articular cartilage keratan sulphates using hydrazinolysis and nitrous acid. Environment of fucose residues.

Brown, Gavin and Huckerby, T. N. and Morris, H. G. and Nieduszynski, I. A. (1992) Degradation of articular cartilage keratan sulphates using hydrazinolysis and nitrous acid. Environment of fucose residues. Biochemical Journal, 286 (1). pp. 235-241. ISSN 1470-8728

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Abstract

Alkaline borohydride-reduced keratan sulphate (KS) chains from bovine articular cartilage (6-8-year-old animals) were fragmented by an anhydrous hydrazine/nitrous acid procedure, previously used on KS by Hopwood & Elliott to isolate the major disaccharides from the poly-N-acetyl-lactosamine repeat sequence [Hopwood & Elliott (1983) Carbohydr. Res. 117, 263-274]. The resulting oligosaccharides were reduced with NaB3H4 or NaBH4 and subjected to ion-exchange chromatography on a Nucleosil 5SB column. In addition to the major disaccharides, two fucose-containing oligosaccharides were examined by high-field 1H n.m.r. spectroscopy, and shown to have the following structures (where AnManOH is 2,5-anhydro-D-mannitol): [formula: see text] It is evident that the presence of fucose protects the N-acetylglucosamine residue from de-N-acetylation, and therefore fragments are produced which preserve the immediate environment of the fucose residue. It may be of biosynthetic significance that these two oligosaccharides contain an unsulphated galactose on the non-reducing side of the fucose residue. The hydrazine/nitrous acid/NaB3H4 method followed by h.p.l.c. provides a sensitive fingerprinting technique for the assay of KS composition and sub-populations.

Item Type: Article
Journal or Publication Title: Biochemical Journal
Uncontrolled Keywords: Keratane sulfate ; Structural analysis ; Chemical method ; NMR spectrometry ; Articular cartilage ; Bovine ; Artiodactyla ; Ungulata ; Mammalia ; Vertebrata
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
Departments: VC's Office
Faculty of Health and Medicine > Biomedical & Life Sciences
Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 22744
Deposited By: ep_ss_importer
Deposited On: 13 Jan 2009 16:38
Refereed?: No
Published?: Published
Last Modified: 26 Jul 2012 16:05
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/22744

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