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Phosphatidylinositol 4,5-bisphosphate specific phospholipase C in Pharbitis nil membranes.

Bonner, P. L. R. and Prior, S. and Hetherington, A. M. and Lumsden, P. J. (1992) Phosphatidylinositol 4,5-bisphosphate specific phospholipase C in Pharbitis nil membranes. Biologia Plantarum, 34 (5-6). pp. 367-372. ISSN 0006-3134

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Abstract

Phosphatidylinositol 4,5-bisphosphate specific phospholipase C has been detected in a membrane preparation fromPharbitis nil cotyledons. The enzyme has a pH optimum of 6.8 and activated by calcium ions, deoxycholate, phosphatidylinositol and phosphatidylethanolamine. The enzyme is inhibited to varying degrees by Tween 20, Triton XI00, zinc, copper, cobalt and manganese ions and phosphatidylserine. G-protein activators do not affect the activity ofPharbitis nil phospholipase C. Analysis of the products of the reaction by HPLC shows inositol 1,4,5-trisphosphate from phospholipase C and inositol bisphosphate from inositol-1 and -5 phosphatase activity.

Item Type: Article
Journal or Publication Title: Biologia Plantarum
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
Departments: Faculty of Science and Technology > Engineering
ID Code: 22743
Deposited By: ep_ss_importer
Deposited On: 13 Jan 2009 16:19
Refereed?: No
Published?: Published
Last Modified: 26 Jul 2012 16:05
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/22743

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