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Oriented degradation of the genome of cyanophage AN-23 after exposure to NaCl.

Bancroft, I. and Smith, R. J. (1992) Oriented degradation of the genome of cyanophage AN-23 after exposure to NaCl. New Phytologist, 121 (4). pp. 571-575. ISSN 1469-8137

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Abstract

The low tolerance of cyanophage AN-23 to NaCl at more than 0·05 M has been investigated. When isolated from clarified lysates that had been exposed to NaCl in the presence of added DNAse I, the phage genomic DNA consisted of a series of discrete fragments. The fragments were recovered from low melting temperature agarose electrophoresis gels and used for restriction analysis. Comparison of the restriction cleavage products with a restriction map of the 46 kbp AN-23 genome has shown that the fragments are generated by removal of DNA from one end of the linear genome in steps of about 3 kbp. The data are consistent with a model in which NaCl triggers the ejection of the phage genome from the capsid.

Item Type: Article
Journal or Publication Title: New Phytologist
Uncontrolled Keywords: Cyanobacterial virus • cyanophage AN-23 • NaCl-promoted degradation
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
Departments: Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 22737
Deposited By: ep_ss_importer
Deposited On: 14 Jan 2009 13:29
Refereed?: No
Published?: Published
Last Modified: 26 Jul 2012 16:05
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/22737

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