Bancroft, I. and Smith, R. J. (1992) Oriented degradation of the genome of cyanophage AN-23 after exposure to NaCl. New Phytologist, 121 (4). pp. 571-575. ISSN 1469-8137Full text not available from this repository.
The low tolerance of cyanophage AN-23 to NaCl at more than 0·05 M has been investigated. When isolated from clarified lysates that had been exposed to NaCl in the presence of added DNAse I, the phage genomic DNA consisted of a series of discrete fragments. The fragments were recovered from low melting temperature agarose electrophoresis gels and used for restriction analysis. Comparison of the restriction cleavage products with a restriction map of the 46 kbp AN-23 genome has shown that the fragments are generated by removal of DNA from one end of the linear genome in steps of about 3 kbp. The data are consistent with a model in which NaCl triggers the ejection of the phage genome from the capsid.
|Journal or Publication Title:||New Phytologist|
|Uncontrolled Keywords:||Cyanobacterial virus • cyanophage AN-23 • NaCl-promoted degradation|
|Subjects:||G Geography. Anthropology. Recreation > GE Environmental Sciences|
|Departments:||Faculty of Science and Technology > Lancaster Environment Centre|
|Deposited On:||14 Jan 2009 13:29|
|Last Modified:||26 Jul 2012 16:05|
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