Bancroft, I. and Smith, R. J. (1992) Oriented degradation of the genome of cyanophage AN-23 after exposure to NaCl. New Phytologist, 121 (4). pp. 571-575. ISSN 1469-8137
Full text not available from this repository.Abstract
The low tolerance of cyanophage AN-23 to NaCl at more than 0·05 M has been investigated. When isolated from clarified lysates that had been exposed to NaCl in the presence of added DNAse I, the phage genomic DNA consisted of a series of discrete fragments. The fragments were recovered from low melting temperature agarose electrophoresis gels and used for restriction analysis. Comparison of the restriction cleavage products with a restriction map of the 46 kbp AN-23 genome has shown that the fragments are generated by removal of DNA from one end of the linear genome in steps of about 3 kbp. The data are consistent with a model in which NaCl triggers the ejection of the phage genome from the capsid.
| Item Type: | Article |
|---|---|
| Journal or Publication Title: | New Phytologist |
| Uncontrolled Keywords: | Cyanobacterial virus • cyanophage AN-23 • NaCl-promoted degradation |
| Subjects: | G Geography. Anthropology. Recreation > GE Environmental Sciences |
| Departments: | Faculty of Science and Technology > Lancaster Environment Centre |
| ID Code: | 22737 |
| Deposited By: | ep_ss_importer |
| Deposited On: | 14 Jan 2009 13:29 |
| Refereed?: | No |
| Published?: | Published |
| Last Modified: | 26 Jul 2012 16:05 |
| Identification Number: | |
| URI: | http://eprints.lancs.ac.uk/id/eprint/22737 |
Actions (login required)
| View Item |
