Mahmod, S. M. and Huddart, H. (1993) Purinergic modulation of spontaneous activity and of responses to high potassium and acetylcholine in rat ileal smooth muscle. Comparative Biochemistry and Physiology Part C: Pharmacology, Toxicology and Endocrinology, 106 (1). pp. 79-85. ISSN 0742-8413
Full text not available from this repository.Abstract
1. In rat ileal smooth muscle both adenosine and ATP at 10−4 M significantly enhanced spontaneous mechanical activity. The excitatory actions of adenosine were blocked by the P1 receptor antagonist 8-phenyltheophylline and the excitatory effects of ATP were significantly reduced by the P2 receptor antagonist quinidine. 2. The P2 receptor desensitizer α,β-methylene-ATP was without effect on ACh responses nor did the stable analogue β,gg-methylene-ATP exert any effect on spontaneous mechanical activity. 3. Pretreatment with adenosine caused a dose-dependent enhancement of K-induced contractures in the ileum. Low adenosine concentrations slightly inhibited and high concentrations slightly enhanced ACh-induced contractures in the ileum. 4. ATP potentiated the phasic component of the ileal K-induced contracture but strongly inhibited tonic force at high concentrations. This agent slightly inhibited the phasic component of the ACh-induced contracture while strongly inhibiting ACh-induced tonic force. 5. α,β-methylene-ATP inhibited ileal muscle ACh induced contractures while it potentiated both phasic and tonic K-induced contractures. β, γ-methylene ATP inhibited ACh-induced contractures but it enhanced K-induced phasic contractures while inhibiting K-induced tonic force. 6. The results of this study suggest that rat ileum may contain the A1 subtype of the P1 receptor but the evidence for a P2 receptor subtype is conflicting despite the inhibition of ATP actions by quinidine. 7. The inhibition of K- and ACh-induced tonic force suggests that adenosine and ATP interactions with ileal smooth muscle may inactivate slow voltage-dependent calcium channels leading to EC uncoupling.
| Item Type: | Article |
|---|---|
| Journal or Publication Title: | Comparative Biochemistry and Physiology Part C: Pharmacology, Toxicology and Endocrinology |
| Uncontrolled Keywords: | Rat ; Ileum ; Smooth muscle ; Spontaneous physical activity ; Nervous control ; Purinergic transmission ; Adenosine ; ATP ; Adenosine receptor ; Potassium ; Acetylcholine ; Excitation contraction coupling ; Inhibition ; Small intestine ; Digestive system ; Rodentia ; Mammalia ; Vertebrata |
| Subjects: | G Geography. Anthropology. Recreation > GE Environmental Sciences |
| Departments: | UNSPECIFIED |
| ID Code: | 22655 |
| Deposited By: | ep_ss_importer |
| Deposited On: | 20 Jan 2009 16:45 |
| Refereed?: | No |
| Published?: | Published |
| Last Modified: | 26 Jul 2012 16:04 |
| Identification Number: | |
| URI: | http://eprints.lancs.ac.uk/id/eprint/22655 |
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