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A bacterially expressed single-chain Fv construct from the 2B4 T-cell receptor.

Kurucz, I. and Jost, C. R. and George, A. J. T. and Andrew, S. M. and Segal, D. M. (1993) A bacterially expressed single-chain Fv construct from the 2B4 T-cell receptor. Proceedings of the National Academy of Sciences, 90 (9). pp. 3830-3834. ISSN 0027-8424

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Abstract

A single-chain Fv construct of the 2B4 T-cell receptor has been made and expressed in Escherichia coli as bacterial inclusion bodies. After solubilization in 6 M guanidine hydrochloride and formation of mixed disulfides with glutathione, the protein was refolded by diluting out the denaturant and allowing intramolecular disulfide bridges to form by disulfide exchange. Approximately 65-100 mg of refolded protein was obtained from 1 liter of bacterial culture, an appreciable fraction of which was monomeric in nondenaturing solvents. This protein bound to three monoclonal antibodies specific for allotypic or idiotypic determinants on the native 2B4 variable region but did not bind several other anti-T-cell-receptor monoclonal antibodies that lacked such specificity. These experiments show that T-cell-receptor variable regions, like the V regions of antibodies, can form a well-behaved single-chain Fv molecule and provide large amounts of recombinant single-chain Fv T-cell receptor that can be used to study T-cell function.

Item Type: Article
Journal or Publication Title: Proceedings of the National Academy of Sciences
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
Departments: Faculty of Health and Medicine > Health Research
ID Code: 22644
Deposited By: ep_ss_importer
Deposited On: 21 Jan 2009 14:35
Refereed?: No
Published?: Published
Last Modified: 01 Oct 2013 10:33
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/22644

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