Onek, Leonzio A. and Lea, Peter J. and Smith, R. John (1994) Isolation and characterization of a calmodulin-like protein from the cyanobacterium Nostoc sp. PCC 6720. Archives of Microbiology, 161 (4). pp. 352-358. ISSN 0302-8933Full text not available from this repository.
A 21-kDa novel polypeptide which possesses characteristics normally considered to be diagnostic of the calmodulin present in eukaryotic cells was isolated from the cyanobacterium Nostoc sp. PCC 6720. The major technique employed in the isolation of the polypeptide was ion-exchange chromatography on a Mono Q column. The 21-kDa polypeptide was shown: to activate pea NAD kinase in vitro, in a Ca2+ requiring reaction; to react with polyclonal antibodies raised against spinach calmodulin, but not with those raised against bovine brain calmodulin; and to exhibit a Ca2+ dependent shift in migration during SDS-PAGE.
|Journal or Publication Title:||Archives of Microbiology|
|Uncontrolled Keywords:||Calcium - binding protein - Calmodulin - Nostoc sp. PCC 6720|
|Subjects:||G Geography. Anthropology. Recreation > GE Environmental Sciences|
|Departments:||Faculty of Science and Technology > Lancaster Environment Centre|
|Deposited On:||28 Jan 2009 16:07|
|Last Modified:||07 Jan 2015 13:39|
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