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Isolation and characterization of a calmodulin-like protein from the cyanobacterium Nostoc sp. PCC 6720.

Onek, Leonzio A. and Lea, Peter J. and Smith, R. John (1994) Isolation and characterization of a calmodulin-like protein from the cyanobacterium Nostoc sp. PCC 6720. Archives of Microbiology, 161 (4). pp. 352-358. ISSN 0302-8933

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Abstract

A 21-kDa novel polypeptide which possesses characteristics normally considered to be diagnostic of the calmodulin present in eukaryotic cells was isolated from the cyanobacterium Nostoc sp. PCC 6720. The major technique employed in the isolation of the polypeptide was ion-exchange chromatography on a Mono Q column. The 21-kDa polypeptide was shown: to activate pea NAD kinase in vitro, in a Ca2+ requiring reaction; to react with polyclonal antibodies raised against spinach calmodulin, but not with those raised against bovine brain calmodulin; and to exhibit a Ca2+ dependent shift in migration during SDS-PAGE.

Item Type: Article
Journal or Publication Title: Archives of Microbiology
Uncontrolled Keywords: Calcium - binding protein - Calmodulin - Nostoc sp. PCC 6720
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
Departments: Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 22500
Deposited By: ep_ss_importer
Deposited On: 28 Jan 2009 16:07
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 16:01
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/22500

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