Odden, Delphine M. and Diatloff, Eugene and Roberts, Stephen K. (2007) A CLC chloride channel plays an essential role in copper homeostasis in Aspergillus nidulans at increased extracellular copper concentrations. Biochimica et Biophysica Acta (BBA) - Biomembranes, 1768 (10). pp. 2466-2477. ISSN 0005-2736Full text not available from this repository.
A putative CLC voltage-gated anion channel gene from Aspergillus nidulans (AnCLCA) is characterised. The expression of the AnCLCA cDNA restored the iron-limited growth of the Saccharomyces cerevisiae CLC null mutant strain (gef1) suggesting that AnCLCA functions as a chloride channel. An AnCLCA conditional mutant was created and exhibited a strong and specific growth inhibition in the presence of extracellular copper concentrations > 18 μM. This sensitivity was shown to be the result of a hyper-accumulation of copper by the conditional mutant, which generates superoxide to toxic levels inhibiting the growth. Further analysis revealed that copper dependent enzymes were disrupted in the AnCLCA conditional null mutant, specifically, a reduced activity of the copper–zinc superoxide dismutase (CuZn–SOD) and enhanced activity of the cytochrome oxidase (COX). These results suggest that AnCLCA plays a key role in copper homeostasis in A. nidulans and that a malfunction of this chloride channel results in disrupted intracellular copper trafficking.
|Journal or Publication Title:||Biochimica et Biophysica Acta (BBA) - Biomembranes|
|Additional Information:||RAE_import_type : Journal article RAE_uoa_type : Earth Systems and Environmental Sciences|
|Uncontrolled Keywords:||CLC ; Chloride channel ; Aspergillus nidulans ; Copper homeostasis ; Filamentous fungi|
|Subjects:||Q Science > QH Natural history > QH301 Biology|
|Departments:||Faculty of Health and Medicine > Biomedical & Life Sciences|
|Deposited On:||05 Feb 2010 14:48|
|Last Modified:||26 Jul 2012 16:01|
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