Cartman, Michelle L. and Morris, James A. and Oates, Kenneth and Huddart, Henry and Staff, William G. (1994) Electrolyte Levels in Neoplastic and Nonneoplastic Human Urothelium After Ouabain Treatment: X-Ray Microanalysis of Bulk Hydrated Samples. Ultrastructural Pathology, 18 (5). pp. 461-466.
Full text not available from this repository.Abstract
Samples of neoplastic and nonneoplastic human urothelium were immediately frozen, incubated in Krebs' saline and then frozen, or incubated in 10-5 mol/L ouabain in Krebs' saline and then frozen. The frozen specimens were then planed in a cryoultramicrotome and examined by low-temperature scanning electron microscopy. X-Ray microanalysis was performed on the superficial urothelial cells. Neoplastic cells immediately frozen and those incubated in Krebs' saline had significantly higher K+/Na+, K+/P, and K+/Cl- ratios and lower Na+/P and Cl-/P ratios than nonneoplastic cells. Incubation in ouabain led to a fall in the K+/Na+, K+/P, and K+/Cl- ratios and a rise in the Na+/P and Na+/Cl- ratios in both neoplastic and nonneoplastic cells and effectively nullified the difference between them. These results are consistent with the concept that in neoplasia a primary event is stimulation of Na+/H+ exchange, which leads to secondary stimulation of the ouabain-sensitive Na+/K+ ATPase pump.
| Item Type: | Article |
|---|---|
| Journal or Publication Title: | Ultrastructural Pathology |
| Uncontrolled Keywords: | low-temperature scanning electron microscopy ; ouabain ; quantitative X-ray microanalysis ; urothelial tumors |
| Subjects: | G Geography. Anthropology. Recreation > GE Environmental Sciences |
| Departments: | Faculty of Science and Technology > Lancaster Environment Centre |
| ID Code: | 22402 |
| Deposited By: | ep_ss_importer |
| Deposited On: | 03 Feb 2009 12:05 |
| Refereed?: | Yes |
| Published?: | Published |
| Last Modified: | 26 Jul 2012 16:00 |
| Identification Number: | |
| URI: | http://eprints.lancs.ac.uk/id/eprint/22402 |
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