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Electrolyte Levels in Neoplastic and Nonneoplastic Human Urothelium After Ouabain Treatment: X-Ray Microanalysis of Bulk Hydrated Samples.

Cartman, Michelle L. and Morris, James A. and Oates, Kenneth and Huddart, Henry and Staff, William G. (1994) Electrolyte Levels in Neoplastic and Nonneoplastic Human Urothelium After Ouabain Treatment: X-Ray Microanalysis of Bulk Hydrated Samples. Ultrastructural Pathology, 18 (5). pp. 461-466.

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Abstract

Samples of neoplastic and nonneoplastic human urothelium were immediately frozen, incubated in Krebs' saline and then frozen, or incubated in 10-5 mol/L ouabain in Krebs' saline and then frozen. The frozen specimens were then planed in a cryoultramicrotome and examined by low-temperature scanning electron microscopy. X-Ray microanalysis was performed on the superficial urothelial cells. Neoplastic cells immediately frozen and those incubated in Krebs' saline had significantly higher K+/Na+, K+/P, and K+/Cl- ratios and lower Na+/P and Cl-/P ratios than nonneoplastic cells. Incubation in ouabain led to a fall in the K+/Na+, K+/P, and K+/Cl- ratios and a rise in the Na+/P and Na+/Cl- ratios in both neoplastic and nonneoplastic cells and effectively nullified the difference between them. These results are consistent with the concept that in neoplasia a primary event is stimulation of Na+/H+ exchange, which leads to secondary stimulation of the ouabain-sensitive Na+/K+ ATPase pump.

Item Type: Article
Journal or Publication Title: Ultrastructural Pathology
Uncontrolled Keywords: low-temperature scanning electron microscopy ; ouabain ; quantitative X-ray microanalysis ; urothelial tumors
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
Departments: Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 22402
Deposited By: ep_ss_importer
Deposited On: 03 Feb 2009 12:05
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 16:00
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/22402

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