Morgan, I. J. and Potts, W. T. W. (1995) The effects of the adrenoceptor agonists phenylephrine and isoproterenol on the intracellular ion concentrations of branchial epithelial-cells of brown trout (Salmo trutta L.). Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology, 165 (6). pp. 458-463. ISSN 0174-1578
Full text not available from this repository.Abstract
Brown trout were fitted with indwelling, intraperitoneal catheters and injected with 4–6 mol · kg-1 of the -receptor agonist phenylephrine or the -receptor agonist isoproterenol. The intracellular concentrations of sodium, chlorine, potassium and phosphorus in the pavement epithelial cells and the mitochondria-rich cells of the branchial epithelium were measured by X-ray microanalysis 1 h after the injection of the adrenoreceptor agonists. Injection with phenylephrine resulted in a significant increase in intracellular chlorine and potassium in mitochondria-rich cells and a significant but relatively smaller increase in chlorine in pavement epithelial cells. Injection with isoproterenol resulted in a significant increase in sodium and chlorine concentration in pavement epithelial cells and a significant decrease in potassium concentration. The only significant effect of isoproterenol injection on mitochondria-rich cells was a decrease in intracellular chlorine concentration. The results suggest that these adrenoreceptor agonists have a direct effect on the influx of Na+ and Cl- across the branchial epithelium. These effects may be a mechanism for acid-base regulation during the severe stress conditions that elicit catecholamine release in vivo. These results corroborate previous studies using X-ray microanalysis which suggested that pavement epithelial cells are the sites of Na+ uptake in freshwater fish whilst Cl- uptake occurs via mitochondria-rich cells.
| Item Type: | Article |
|---|---|
| Journal or Publication Title: | Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology |
| Uncontrolled Keywords: | Gills - X-ray microanalysis - Phenylephrine - Isoproterenol - Trout ; Salmo trutta |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Departments: | Faculty of Science and Technology > Lancaster Environment Centre |
| ID Code: | 22303 |
| Deposited By: | ep_ss_importer |
| Deposited On: | 05 Feb 2009 16:32 |
| Refereed?: | No |
| Published?: | Published |
| Last Modified: | 26 Jul 2012 15:59 |
| Identification Number: | |
| URI: | http://eprints.lancs.ac.uk/id/eprint/22303 |
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