Morgan, I. J. and Potts, W. T. W. (1995) The effects of the adrenoceptor agonists phenylephrine and isoproterenol on the intracellular ion concentrations of branchial epithelial-cells of brown trout (Salmo trutta L.). Journal of Comparative Physiology B, 165 (6). pp. 458-463. ISSN 0174-1578Full text not available from this repository.
Brown trout were fitted with indwelling, intraperitoneal catheters and injected with 4–6 mol · kg-1 of the -receptor agonist phenylephrine or the -receptor agonist isoproterenol. The intracellular concentrations of sodium, chlorine, potassium and phosphorus in the pavement epithelial cells and the mitochondria-rich cells of the branchial epithelium were measured by X-ray microanalysis 1 h after the injection of the adrenoreceptor agonists. Injection with phenylephrine resulted in a significant increase in intracellular chlorine and potassium in mitochondria-rich cells and a significant but relatively smaller increase in chlorine in pavement epithelial cells. Injection with isoproterenol resulted in a significant increase in sodium and chlorine concentration in pavement epithelial cells and a significant decrease in potassium concentration. The only significant effect of isoproterenol injection on mitochondria-rich cells was a decrease in intracellular chlorine concentration. The results suggest that these adrenoreceptor agonists have a direct effect on the influx of Na+ and Cl- across the branchial epithelium. These effects may be a mechanism for acid-base regulation during the severe stress conditions that elicit catecholamine release in vivo. These results corroborate previous studies using X-ray microanalysis which suggested that pavement epithelial cells are the sites of Na+ uptake in freshwater fish whilst Cl- uptake occurs via mitochondria-rich cells.
|Journal or Publication Title:||Journal of Comparative Physiology B|
|Uncontrolled Keywords:||Gills - X-ray microanalysis - Phenylephrine - Isoproterenol - Trout ; Salmo trutta|
|Subjects:||Q Science > QH Natural history > QH301 Biology|
|Departments:||Faculty of Science and Technology > Lancaster Environment Centre|
|Deposited On:||05 Feb 2009 16:32|
|Last Modified:||13 Jan 2016 11:26|
Actions (login required)