McAinsh, M. R. and Webb, A. A. R. and Taylor, J. E. and Hetherington, A. M. (1995) Stimulus-Induced Oscillations in Guard Cell Cytosolic Free Calcium. The Plant Cell, 7 (8). pp. 1207-1219.
Full text not available from this repository.Abstract
Ca2+ is implicated as a second messenger in the response of stomata to a range of stimuli. However, the mechanism by which stimulus-induced increases in guard cell cytosolic free Ca2+ ([Ca2+]i) are transduced into different physiological responses remains to be explained. Oscillations in [Ca2+]i may provide one way in which this can occur. We used photometric and imaging techniques to examine this hypothesis in guard cells of Commelina communis. External Ca2+ ([Ca2+]e), which causes an increase in [Ca2+]i, was used as a closing stimulus. The total increase in [Ca2+]i was directly related to the concentration of [Ca2+]e, both of which correlated closely with the degree of stomatal closure. Increases were oscillatory in nature, with the pattern of the oscillations dependent on the concentration of [Ca2+]e. At 0.1 mM, [Ca2+]e induced symmetrical oscillations. In contrast, 1.0 mM [Ca2+]e induced asymmetric oscillations. Oscillations were stimulus dependent and modulated by changing [Ca2+]e. Experiments using Ca2+ channel blockers and Mn2+-quenching studies suggested a role for Ca2+ influx during the oscillatory behavior without excluding the possible involvement of Ca2+ release from intracellular stores. These data suggest a mechanism for encoding the information required to distinguish between a number of different Ca2+-mobilizing stimuli in guard cells, using stimulus-specific patterns of oscillations in [Ca2+]i.
| Item Type: | Article |
|---|---|
| Journal or Publication Title: | The Plant Cell |
| Subjects: | G Geography. Anthropology. Recreation > GE Environmental Sciences |
| Departments: | Faculty of Science and Technology > Lancaster Environment Centre Faculty of Science and Technology > Engineering |
| ID Code: | 22297 |
| Deposited By: | ep_ss_importer |
| Deposited On: | 06 Feb 2009 13:47 |
| Refereed?: | No |
| Published?: | Published |
| Last Modified: | 26 Jul 2012 15:59 |
| Identification Number: | |
| URI: | http://eprints.lancs.ac.uk/id/eprint/22297 |
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