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Stimulus-Induced Oscillations in Guard Cell Cytosolic Free Calcium.

McAinsh, M. R. and Webb, A. A. R. and Taylor, J. E. and Hetherington, A. M. (1995) Stimulus-Induced Oscillations in Guard Cell Cytosolic Free Calcium. The Plant Cell, 7 (8). pp. 1207-1219. ISSN 1532-298X

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Abstract

Ca2+ is implicated as a second messenger in the response of stomata to a range of stimuli. However, the mechanism by which stimulus-induced increases in guard cell cytosolic free Ca2+ ([Ca2+]i) are transduced into different physiological responses remains to be explained. Oscillations in [Ca2+]i may provide one way in which this can occur. We used photometric and imaging techniques to examine this hypothesis in guard cells of Commelina communis. External Ca2+ ([Ca2+]e), which causes an increase in [Ca2+]i, was used as a closing stimulus. The total increase in [Ca2+]i was directly related to the concentration of [Ca2+]e, both of which correlated closely with the degree of stomatal closure. Increases were oscillatory in nature, with the pattern of the oscillations dependent on the concentration of [Ca2+]e. At 0.1 mM, [Ca2+]e induced symmetrical oscillations. In contrast, 1.0 mM [Ca2+]e induced asymmetric oscillations. Oscillations were stimulus dependent and modulated by changing [Ca2+]e. Experiments using Ca2+ channel blockers and Mn2+-quenching studies suggested a role for Ca2+ influx during the oscillatory behavior without excluding the possible involvement of Ca2+ release from intracellular stores. These data suggest a mechanism for encoding the information required to distinguish between a number of different Ca2+-mobilizing stimuli in guard cells, using stimulus-specific patterns of oscillations in [Ca2+]i.

Item Type: Article
Journal or Publication Title: The Plant Cell
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
Departments: Faculty of Science and Technology > Lancaster Environment Centre
Faculty of Science and Technology > Engineering
ID Code: 22297
Deposited By: ep_ss_importer
Deposited On: 06 Feb 2009 13:47
Refereed?: No
Published?: Published
Last Modified: 09 Apr 2014 20:26
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/22297

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