Huckerby, Thomas N. and Brown, Gavin M. and Nieduszynski, Ian A. (1995) <sup>13</sup>C-NMR spectroscopy of keratan sulphates. European Journal of Biochemistry, 231 (3). pp. 779-783.Full text not available from this repository.
Skeletal keratan sulphate has been fragmented using the enzyme keratanase II, and the complete <sup>13</sup>C chemical shift data are reported for the four tetrasaccharides which derived from the repeat region. They have the structures: Galβ(1–4)GlcNAc(6S)β(1–3)Galβ(1–4)GlcNAc(6S)-ol, Gal(6S)β(1–4)GlcNAc(6S)β(1–3)Gal(1–4)GlcNAc(6S)-ol, Galβ(1–4)GlcNAc(6S)β(1–3)Gal(6S)β(1–4)GlcNAc(6S)-ol, and Gal(6S)β(1–4)GlcNAc(6S)(1–3)Gal(6S)β(1–4)GlcNAc(6S)-ol, where GlcNAc(6S)-ol represents N-acetyl-glucosaminitol-6-O-sulphate. The value of these data for microstructural analysis of undegraded keratan sulphate samples using <sup>13</sup>C-NMR spectroscopy is discussed with respect to galactose sulphation levels and the block sulphation structure.
|Journal or Publication Title:||European Journal of Biochemistry|
|Subjects:||G Geography. Anthropology. Recreation > GE Environmental Sciences|
|Departments:||Faculty of Science and Technology > Lancaster Environment Centre|
Faculty of Health and Medicine > Biomedical & Life Sciences
|Deposited On:||06 Feb 2009 15:12|
|Last Modified:||31 May 2013 11:49|
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