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Electrolyte homeostasis in urothelial neoplasia: the effects of amiloride.

Cartman, M. L. and Morris, J. A. and Huddart, H. and Staff, W. G. (1995) Electrolyte homeostasis in urothelial neoplasia: the effects of amiloride. British Journal of Urology, 75 (5). pp. 599-603.

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Abstract

Objective To investigate the effect of amiloride pre-incubation on the intracellular electrolyte levels in neoplastic and non-neoplastic urothelium. Materials and methods Samples of papillary transitional cell carcinomas (grades 1 and 2) and non-neoplastic human urothelium were either frozen immediately or after pre-incubation with Krebs saline alone or with Krebs saline containing amiloride. The frozen samples were planed in a cryo-ultramicrotome and then examined by low temperature scanning electron microscopy. Intracellular electrolyte levels were measured using X-ray microanalysis. Results In the cells frozen immediately the ratios K+/Na+, K+/P and K+/Cl- were significantly increased in neoplastic compared with non-neoplastic urothelial cells (P<0.001). This difference was also found in the cells pre-incubated with Krebs saline alone but was not present in the cells pre-incubated with amiloride. Conclusions These results were consistent with the concept that activation of the amiloride-sensitive Na+/H + membrane exchanger is a primary event in neoplasia.

Item Type: Article
Journal or Publication Title: British Journal of Urology
Uncontrolled Keywords: Amiloride • LTSEM • Na+/H+ antiport • urothelial neoplasia • X-ray microanalysis
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
Departments: Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 22248
Deposited By: ep_ss_importer
Deposited On: 10 Feb 2009 15:14
Refereed?: No
Published?: Published
Last Modified: 26 Jul 2012 15:58
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/22248

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