Cartman, M. L. and Morris, J. A. and Huddart, H. and Staff, W. G. (1995) Electrolyte homeostasis in urothelial neoplasia: the effects of amiloride. British Journal of Urology, 75 (5). pp. 599-603.Full text not available from this repository.
Objective To investigate the effect of amiloride pre-incubation on the intracellular electrolyte levels in neoplastic and non-neoplastic urothelium. Materials and methods Samples of papillary transitional cell carcinomas (grades 1 and 2) and non-neoplastic human urothelium were either frozen immediately or after pre-incubation with Krebs saline alone or with Krebs saline containing amiloride. The frozen samples were planed in a cryo-ultramicrotome and then examined by low temperature scanning electron microscopy. Intracellular electrolyte levels were measured using X-ray microanalysis. Results In the cells frozen immediately the ratios K+/Na+, K+/P and K+/Cl- were significantly increased in neoplastic compared with non-neoplastic urothelial cells (P<0.001). This difference was also found in the cells pre-incubated with Krebs saline alone but was not present in the cells pre-incubated with amiloride. Conclusions These results were consistent with the concept that activation of the amiloride-sensitive Na+/H + membrane exchanger is a primary event in neoplasia.
|Journal or Publication Title:||British Journal of Urology|
|Uncontrolled Keywords:||Amiloride • LTSEM • Na+/H+ antiport • urothelial neoplasia • X-ray microanalysis|
|Subjects:||G Geography. Anthropology. Recreation > GE Environmental Sciences|
|Departments:||Faculty of Science and Technology > Lancaster Environment Centre|
|Deposited On:||10 Feb 2009 15:14|
|Last Modified:||07 Jan 2015 13:36|
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