Tai, Gui-Hua and Huckerby, Thomas N. and Nieduszynski, Ian A. (1996) Multiple Non-reducing Chain Termini Isolated from Bovine Corneal Keratan Sulfates. Journal of Biological Chemistry, 271 (38). pp. 23535-23546. ISSN 1083-351XFull text not available from this repository.
From the Division of Biological Sciences, Institute of Environmental and Biological Sciences, Lancaster University, Bailrigg, Lancaster LA1 4YQ and The Polymer Centre, School of Physics and Chemistry, Lancaster University, Bailrigg, Lancaster LA1 4YA, United Kingdom Keratan sulfate-containing proteoglycans were isolated from bovine cornea (15-month-old to 3-year-old animals) and digested with the enzyme, keratanase II. The released oligosaccharides, which included non-reducing termini and repeat region oligosaccharides but not linkage regions, were reduced with alkaline borohydride and fractionated on a Spherisorb column. These oligosaccharides were examined by 600-MHz 1H NMR spectroscopy using one- and two-dimensional methods and, in addition to some oligosaccharide alditols previously recovered from skeletal keratan sulfate, the following new capping structures were identified: NeuAc2-6Gal1-4GlcNAc(S)-ol, NeuAc2-3Gal(S)1-4GlcNAc(S)1-3Gal1-4GlcNAc(S)-ol, NeuGc2-6Gal1-4GlcNAc(S)1-3Gal1-4GlcNAc(S)-ol, NeuGc2-3Gal1-4GlcNAc(S)1-3Gal1-4GlcNAc(S)-ol, NeuGc2-3Gal(S)1-4GlcNAc(S)1-3Gal1-4GlcNAc(S)-ol, NeuGc2-3Gal(S)1-4GlcNAc(S)1-3Gal(S)1-4GlcNAc(S)-ol, Gal1-3Gal1-4GlcNAc(S)1-3Gal1-4GlcNAc(S)-ol, Gal1-3Gal1-4GlcNAc(S)1-3Gal(S)1-4GlcNAc(S)-ol, GlcNAc(S)1-3Gal(S)1-4GlcNAc(S)-ol, and GalNAc(S)1-3Gal(S)1-4GlcNAc(S)-ol. These structures represent seven families of capping residues, whose relative molar proportions are given in parentheses: NeuAc(2-3)- (12%), NeuAc(2-6)- (41%), NeuGc(2-3)- and NeuGc(2-6)- families (12%), Gal(1-3)- (26%), GalNAc(S)(1-3)- (5%), and GlcNAc(S)(1-3)- (4%). It is not clear, at present, where each of these structures occurs on the bi-antennary N-linked corneal keratan sulfate chains, which themselves occur within three keratan sulfate proteoglycan species. However, examination of the relative proportions of the capping to the repeat structures and knowledge of the average molecular size suggests that the sum of these non-reducing termini represents the caps of two antennae.
|Journal or Publication Title:||Journal of Biological Chemistry|
|Subjects:||G Geography. Anthropology. Recreation > GE Environmental Sciences|
|Departments:||Faculty of Science and Technology > Lancaster Environment Centre|
|Deposited On:||13 Feb 2009 09:37|
|Last Modified:||04 Nov 2015 00:03|
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