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Cell cycle variation in DNA migration in pulsed field gel electrophoresis.

Mateos, S. and Gordon, A. T. and Steel, G. G. and McMillan, T. J. (1996) Cell cycle variation in DNA migration in pulsed field gel electrophoresis. International Journal of Radiation Biology, 69 (6). pp. 687-694.

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Abstract

Pulsed-field electrophoresis is being used extensively in the gene mapping studies and in the analysis of DNA strand breakage by ionizing radiation. We have evaluated the relationship between the fraction of S phase DNA in a cell population and its ability to modify the migration of DNA in pulsed-field gel electrophoresis. We have shown that increasing the proportion of S phase DNA reduced the effective rate of migration of MGH-U1 cellular DNA. This effect was observed after treatment with ionizing radiation or the restriction enzyme Not I. However, when radiation-induced damage was studied using intact cells, only the DNA with 70 percent S phase showed apparent differences in damage induction. These studies therefore provide data to indicate the percentage of S phase cells at which overall DNA migration might be affected significantly.

Item Type: Article
Journal or Publication Title: International Journal of Radiation Biology
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
Departments: Faculty of Science and Technology > Lancaster Environment Centre
VC's Office
ID Code: 22128
Deposited By: ep_ss_importer
Deposited On: 17 Feb 2009 11:09
Refereed?: No
Published?: Published
Last Modified: 26 Jul 2012 15:56
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/22128

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