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Synthetic phosphooligosaccharide fragments of lipophosphoglycan as acceptors for Leishmania-major alpha-Dmannosylphosphate transferase.

Brown, Gavin M. and Millar, Alan R. and Masterson, Claire and Brimacombe, John S. and Nikolaev, Andrei V. and Ferguson, Michael A. (1996) Synthetic phosphooligosaccharide fragments of lipophosphoglycan as acceptors for Leishmania-major alpha-Dmannosylphosphate transferase. European Journal of Biochemistry, 242 (2). pp. 410-416. ISSN 0014-2956

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Abstract

Protozoan parasites of the genus Leishmania synthesise lipophosphoglycans, phosphoglycans and proteophosphoglycans that contain phosphosaccharide-repeat units of [-6Galβ1-4Manα1-P-]. In this study, a GDP-Man-dependent α-mannosylphosphate-transferase activity was detected in washed Leishmania major membranes using synthetic phospho-oligosaccharide fragments of lipophosphoglycan as acceptor substrates. The divalent-cation-dependent α-mannosylphosphate-transferase activity had an apparent Km for GDP-Man of about 15–20 μM and a pH optimum of 7.0. The activity showed a requirement for a non-reducing terminal βGal residue and for one or more phosphodiester units preceding the acceptor site. Based on these results, the activity may be defined as a GDP-Man:Galβ1-4Manα1-P-R α-mannosylphosphate-transferase. This acceptor specificity is consistent with a role for the α-mannosylphosphate transferase in the elongation of phosphosaccharide-repeat domains of Leishmania glycoconjugates rather than in the priming of these domains. An identical or similar activity must exist in the amastigote forms of the Leishmania that produce and secrete proteophosphoglycan material and the activity therefore represents a feasible target for the development of chemotherapeutics.

Item Type: Article
Journal or Publication Title: European Journal of Biochemistry
Uncontrolled Keywords: lipophosphoglycan • proteophosphoglycan • Leishmania • phospho-oligosaccharide biosynthesis
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
VC's Office
ID Code: 22003
Deposited By: ep_ss_importer
Deposited On: 17 Feb 2009 09:36
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 15:54
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/22003

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