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Characterisation of a nonreducing terminal fragment from bovine articular-cartilage keratan sulfates containing alpha(2-3)-linked sialic-acid and alpha(1-3)-linked fucose.

Brown, Gavin M. and Huckerby, T. N. and Abram, B. L. and Nieduszynski, I. A. (1996) Characterisation of a nonreducing terminal fragment from bovine articular-cartilage keratan sulfates containing alpha(2-3)-linked sialic-acid and alpha(1-3)-linked fucose. Biochemical Journal, 319 (1). pp. 137-141. ISSN 0264-6021

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Abstract

Alkaline-borohydride-reduced keratan sulphate chains were isolated from bovine articular cartilage (6–8-year-old animals) and digested with keratanase II, an endo-b-N-acetylglucosaminidase. The resulting oligosaccharides were borohydride-reduced and fractionated on a strong anion-exchange column. 1H-NMR spectroscopic analysis of the products revealed one containing both a(2-3)-linked sialic acid and a(1-3)-linked fucose which was shown to have the structure (I) shown. This structure is a sulphated variant of the VIM-2 epitope (CD65), a putative ligand of E-selectin. No oligosaccharide containing the sialyl-Lex structure [NeuAca2-3Galb1-4(Fuca1-3)GlcNAcb1-] was identified in this study.

Item Type: Article
Journal or Publication Title: Biochemical Journal
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
VC's Office
Faculty of Science and Technology > Lancaster Environment Centre
Faculty of Science and Technology > Psychology
Faculty of Science and Technology > Mathematics and Statistics
ID Code: 22002
Deposited By: ep_ss_importer
Deposited On: 17 Feb 2009 09:41
Refereed?: No
Published?: Published
Last Modified: 26 Jul 2012 15:54
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/22002

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