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The impact of ozone, isoprene and propene on antioxidant levels in two leaf classes of velvet beans (Mucana pruriens L.).

Stokes, N. J. and Terry, G. T. and Hewitt, CN (1998) The impact of ozone, isoprene and propene on antioxidant levels in two leaf classes of velvet beans (Mucana pruriens L.). Journal of Experimental Botany, 49 (318). pp. 115-123. ISSN 1460-2431

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Abstract

Four-week-old velvet beans (Mucuna pruriens L.) were fumigated with ozone, isoprene and propene both singly and in combination (hydrocarbon+oxidant) in controlled-environment chambers for a 4 week period. Measurements were made of total soluble protein, ascorbate, dehydroascorbate, lipid peroxidation, and glutathione reductase activity in mature and young velvet bean leaves.Significant increases in soluble protein concentrations with respect to the controls were found in plants fumigated with propene and isoprene+ozone for young leaves, and ozone only for mature leaves. The analysis of ascorbate concentrations in young leaves showed a significant increase in total ascorbate for propene-fumigated plants a significant decrease in the level of the oxidized form (dehydroascorbate) for ozone and isoprene+ozone-treated plants, and a significant increase in the reduced form for plants fumigated with propene, propene+ozone and isoprene+ozone. The analysis of ascorbate levels in mature leaves showed no significant effect from any fumigation regime.When compared to control material the specific activity of the enzyme glutathione reductase was found to be significantly reduced in young leaves treated with propene, isoprene+ozone and ozone alone. However, in mature leaves this effect is lost and there are no significant differences with respect to the control

Item Type: Article
Journal or Publication Title: Journal of Experimental Botany
Uncontrolled Keywords: Ozone ; hydrocarbons ; velvet bean ; Mucuna pruriens ; antioxidants.
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
Departments: Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 21871
Deposited By: ep_ss_importer
Deposited On: 10 Feb 2009 16:53
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 15:52
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/21871

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