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Can microbial mineralization be used to estimate microbial availability of organic contaminants in soil?

Semple, Kirk T. and Dew, Nadia M. and Doick, Kieron J. and Rhodes, Angela (2006) Can microbial mineralization be used to estimate microbial availability of organic contaminants in soil? Environmental Pollution, 140 (1). pp. 164-172. ISSN 0269-7491

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Abstract

The aim of this study was to characterize the behaviour of a PAH-degrading bacterium to determine whether mineralization plateaus as a result of substrate removal, a decrease in microbial activity or nutrient availability in sterile soils over time. To investigate this, the mineralization of 14C-phenanthrene was measured until it plateaued; subsequently, additional 14C-phenanthrene, catabolic inocula or nutrients were introduced and mineralization was measured for a further 10 d. Cell numbers were also measured together with 14C-uptake into microbial biomass. Freshly added 14C-phenanthrene was rapidly metabolised by the microorganisms. Neither the addition of a catabolic inoculum nor nutrients affected the extent of 14C-phenanthrene mineralization. Cell numbers remained constant over time, with only a small amount of the 14C-activity incorporated into the microbial biomass. This study indicated that the termination of mineralization was due to the removal of available phenanthrene and not decreasing cellular activity or cell death. The mineralization values also correlated with 14C-phenanthrene extractability using β-cyclodextrin. Mineralization can estimate the microbial availability of 14C-contaminants in soil.

Item Type: Article
Journal or Publication Title: Environmental Pollution
Additional Information: Semple was the senior and corresponding author of this paper. Dew/Doick/ Rhodes were postgraduate students, supervised by Semple. Numerous studies have assumed that mineralisation of chemicals is a measure of bioavailability and as a comparator for its chemical determination (see Paper 1 above); this paper addressed this assumption. RAE_import_type : Journal article RAE_uoa_type : Earth Systems and Environmental Sciences
Uncontrolled Keywords: Phenanthrene ; Bioavailability ; Respirometry ; HPCD ; Microbial inoculum
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
Departments: Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 2143
Deposited By: ep_importer
Deposited On: 07 Apr 2008 16:50
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 15:46
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/2143

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