Temporal changes in the availability of pyrene in two soils under sterile and non-sterile conditions.

Macleod, Christopher J. A. and Semple, Kirk T. (2003) Temporal changes in the availability of pyrene in two soils under sterile and non-sterile conditions. Soil Biology and Biochemistry, 35 (11). pp. 1443-1450. ISSN 0038-0717

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Abstract

In this study, temporal changes in the extractability of 14C-pyrene, at native concentrations, were followed in two soils with differing organic matter contents, under sterile and non-sterile conditions over 24 weeks by a sequential solvent extraction scheme. No significant loss of the added 14C-pyrene was observed during the incubation. Significant decreases in methanol:water and n-butanol extractability were observed with increasing soil–pyrene contact time. Significant non-extractable residues were formed in all soils, with the largest increases found in the non-sterile soils. After 8 weeks soil–pyrene contact time, there was a significant increase in the rate and extent of sequestration of pyrene in the biologically active soils. This indicated that the aging of pyrene was initially a physical process, with active microbial communities increasing the rate and extent of residue formation after 8 weeks soil–pyrene contact time. These findings suggest that there is a need for longer term ageing experiments following the role of microbial communities on the formation of solvent non-extractable residues. The humin fraction of the soil organic matter contained the majority of the 14C-pyrene associated activity which was not extractable using the scheme of sequential solvents. Saponification of the soil humin resulted in the release of similar amounts of 14C-pyrene associated activity from sterile and non-sterile soils. Solvent extraction with methanol:water was found to significantly underestimate the bioavailable fraction, whereas n-butanol overestimated the bioavailability of the 14C-pyrene-associated activity when assessed by bacterial mineralization after 24 weeks soil–pyrene contact time.

Item Type:
Journal Article
Journal or Publication Title:
Soil Biology and Biochemistry
Uncontrolled Keywords:
/dk/atira/pure/researchoutput/libraryofcongress/ge
Subjects:
?? SEQUENTIAL SOLVENT EXTRACTIONBIOAVAILABILITYNON-EXTRACTABLE RESIDUESMICROBIAL ACTIVITYSOIL SCIENCEMICROBIOLOGYGE ENVIRONMENTAL SCIENCES ??
ID Code:
21403
Deposited By:
Deposited On:
13 Jan 2009 11:24
Refereed?:
Yes
Published?:
Published
Last Modified:
11 Sep 2023 12:42