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The GreenScreen genotoxicity assay : a screening validation programme.

Cahill, P. A. and Knight, A. W. and Billinton, N. and Barker, M. G. and Walsh, L. and Keenan, P. O. and Williams, C. V. and Tweats, D. J. and Walmsley, R. M. (2004) The GreenScreen genotoxicity assay : a screening validation programme. Mutagenesis, 19 (2). pp. 105-119. ISSN 1464-3804

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Abstract

A yeast (Saccharomyces cerevisiae) DNA repair reporter assay termed the GreenScreen® assay (GSA) is described. This is a novel, cost-effective genotoxicity screen, developed to provide a pre-regulatory screening assay for use by the pharmaceutical industry and in other applications where significant numbers of compounds need to be tested. It provides a higher throughput and a lower compound consumption than existing eukaryotic genotoxicity assays and is sensitive to a broad spectrum of mutagens and, importantly, clastogens. We describe a simple, robust assay protocol and a validation study. The end-point of the test reflects the typically eukaryotic chromosomes and DNA metabolizing enzymes of yeast. The capacity for metabolic activation (MA) in yeast is limited compared with the mammalian liver or its extracts, but the assay does detect a subset of compounds that would require MA in existing genotoxicity tests. The GSA detects a different spectrum of compounds to bacterial genotoxicity assays and thus, together with an in silico structure–activity relationship (SAR) screen, and possibly a high throughput bacterial screen, would provide an effective preview of the regulatory battery of genotoxicity tests.

Item Type: Article
Journal or Publication Title: Mutagenesis
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
Departments: Faculty of Science and Technology > Lancaster Environment Centre
Faculty of Health and Medicine > Health Research
ID Code: 21303
Deposited By: ep_ss_importer
Deposited On: 08 Jan 2009 15:03
Refereed?: No
Published?: Published
Last Modified: 26 Jul 2012 15:45
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/21303

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